Zero-mode waveguides for single molecule analysis and fast DNA sequencing

Michael Levene; Jonas Korlach; Stephen Turner; Harold G. Craighead; Watt W. Webb

Zero-mode waveguides for single molecule analysis and fast DNA sequencing

Michael Levene, Jonas Korlach, Stephen Turner, Harold G. Craighead, Watt W. Webb

Houston Methodist

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

Zero-mode waveguides for single molecule analysis and fast DNA sequencing were presented. Arrays of zero-mode waveguides which consisted of subwavelengths holes in thin metal films on fused silica coverslips were manufactured using electron-beam lithography. A finite-element model of intensity distribution within such waveguides was used to predict the effective observation volume in the waveguides. The effectiveness of zero-mode waveguides was demonstrated by real-time observation of single molecules of DNA polymerase immobilized inside waveguides.

Original language	English
Title of host publication	Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest
State	Published - Jan 1 2002
Event	Conference on Lasers and Electro-Optics (CLEO 2002) - Long Beach, CA, United States Duration: May 19 2002 → May 24 2002

Other

Other	Conference on Lasers and Electro-Optics (CLEO 2002)
Country/Territory	United States
City	Long Beach, CA
Period	5/19/02 → 5/24/02

ASJC Scopus subject areas

General Physics and Astronomy

Cite this

@inproceedings{8bbaad535f374720a11166e354f6bde6,

title = "Zero-mode waveguides for single molecule analysis and fast DNA sequencing",

abstract = "Zero-mode waveguides for single molecule analysis and fast DNA sequencing were presented. Arrays of zero-mode waveguides which consisted of subwavelengths holes in thin metal films on fused silica coverslips were manufactured using electron-beam lithography. A finite-element model of intensity distribution within such waveguides was used to predict the effective observation volume in the waveguides. The effectiveness of zero-mode waveguides was demonstrated by real-time observation of single molecules of DNA polymerase immobilized inside waveguides.",

author = "Michael Levene and Jonas Korlach and Stephen Turner and Craighead, {Harold G.} and Webb, {Watt W.}",

year = "2002",

month = jan,

day = "1",

language = "English",

booktitle = "Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest",

note = "Conference on Lasers and Electro-Optics (CLEO 2002) ; Conference date: 19-05-2002 Through 24-05-2002",

}

TY - GEN

T1 - Zero-mode waveguides for single molecule analysis and fast DNA sequencing

AU - Levene, Michael

AU - Korlach, Jonas

AU - Turner, Stephen

AU - Craighead, Harold G.

AU - Webb, Watt W.

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Zero-mode waveguides for single molecule analysis and fast DNA sequencing were presented. Arrays of zero-mode waveguides which consisted of subwavelengths holes in thin metal films on fused silica coverslips were manufactured using electron-beam lithography. A finite-element model of intensity distribution within such waveguides was used to predict the effective observation volume in the waveguides. The effectiveness of zero-mode waveguides was demonstrated by real-time observation of single molecules of DNA polymerase immobilized inside waveguides.

AB - Zero-mode waveguides for single molecule analysis and fast DNA sequencing were presented. Arrays of zero-mode waveguides which consisted of subwavelengths holes in thin metal films on fused silica coverslips were manufactured using electron-beam lithography. A finite-element model of intensity distribution within such waveguides was used to predict the effective observation volume in the waveguides. The effectiveness of zero-mode waveguides was demonstrated by real-time observation of single molecules of DNA polymerase immobilized inside waveguides.

UR - http://www.scopus.com/inward/record.url?scp=0036455541&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036455541&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:0036455541

BT - Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest

T2 - Conference on Lasers and Electro-Optics (CLEO 2002)

Y2 - 19 May 2002 through 24 May 2002

ER -

Zero-mode waveguides for single molecule analysis and fast DNA sequencing

Abstract

Other

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this