Zero-mode waveguides for single molecule analysis and fast DNA sequencing

Michael Levene; Jonas Korlach; Stephen Turner; Harold G. Craighead; Watt W. Webb

Zero-mode waveguides for single molecule analysis and fast DNA sequencing

Michael Levene, Jonas Korlach, Stephen Turner, Harold G. Craighead, Watt W. Webb

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Zero-mode waveguides for single molecule analysis and fast DNA sequencing were presented. Arrays of zero-mode waveguides which consisted of subwavelengths holes in thin metal films on fused silica coverslips were manufactured using electron-beam lithography. A finite-element model of intensity distribution within such waveguides was used to predict the effective observation volume in the waveguides. The effectiveness of zero-mode waveguides was demonstrated by real-time observation of single molecules of DNA polymerase immobilized inside waveguides.

Original language	English
Title of host publication	Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest
State	Published - Jan 1 2002
Event	Conference on Lasers and Electro-Optics (CLEO 2002) - Long Beach, CA, United States Duration: May 19 2002 → May 24 2002

Other

Other	Conference on Lasers and Electro-Optics (CLEO 2002)
Country/Territory	United States
City	Long Beach, CA
Period	5/19/02 → 5/24/02

ASJC Scopus subject areas

Physics and Astronomy(all)

Cite this

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title = "Zero-mode waveguides for single molecule analysis and fast DNA sequencing",

abstract = "Zero-mode waveguides for single molecule analysis and fast DNA sequencing were presented. Arrays of zero-mode waveguides which consisted of subwavelengths holes in thin metal films on fused silica coverslips were manufactured using electron-beam lithography. A finite-element model of intensity distribution within such waveguides was used to predict the effective observation volume in the waveguides. The effectiveness of zero-mode waveguides was demonstrated by real-time observation of single molecules of DNA polymerase immobilized inside waveguides.",

author = "Michael Levene and Jonas Korlach and Stephen Turner and Craighead, {Harold G.} and Webb, {Watt W.}",

year = "2002",

month = jan,

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T1 - Zero-mode waveguides for single molecule analysis and fast DNA sequencing

AU - Levene, Michael

AU - Korlach, Jonas

AU - Turner, Stephen

AU - Craighead, Harold G.

AU - Webb, Watt W.

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Zero-mode waveguides for single molecule analysis and fast DNA sequencing were presented. Arrays of zero-mode waveguides which consisted of subwavelengths holes in thin metal films on fused silica coverslips were manufactured using electron-beam lithography. A finite-element model of intensity distribution within such waveguides was used to predict the effective observation volume in the waveguides. The effectiveness of zero-mode waveguides was demonstrated by real-time observation of single molecules of DNA polymerase immobilized inside waveguides.

AB - Zero-mode waveguides for single molecule analysis and fast DNA sequencing were presented. Arrays of zero-mode waveguides which consisted of subwavelengths holes in thin metal films on fused silica coverslips were manufactured using electron-beam lithography. A finite-element model of intensity distribution within such waveguides was used to predict the effective observation volume in the waveguides. The effectiveness of zero-mode waveguides was demonstrated by real-time observation of single molecules of DNA polymerase immobilized inside waveguides.

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M3 - Conference contribution

AN - SCOPUS:0036455541

BT - Pacific Rim Conference on Lasers and Electro-Optics, CLEO - Technical Digest

Y2 - 19 May 2002 through 24 May 2002

ER -

Zero-mode waveguides for single molecule analysis and fast DNA sequencing

Abstract

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ASJC Scopus subject areas

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