TY - JOUR
T1 - Zebrafish models of dyslipidemia
T2 - Relevance to atherosclerosis and angiogenesis
AU - Fang, Longhou
AU - Liu, Chao
AU - Miller, Yury I.
N1 - Funding Information:
Work in the authors' laboratory is supported by the NIH grants HL093767 (Y.I.M.), HL055798 (Y.I.M.) and HL114734 (L.F.). All authors have read the journal's policy on conflicts of interest and wish to disclose that Y.M. has received an investigator-initiated grant from Merck and is a co-inventor of a patent owned by the University of California for the use of the hypercholesterolemic zebrafish model. Y.M. and L.F. are co-inventors of a patent owned by the University of California related to therapeutic applications of apoA-I binding protein.
PY - 2014/2
Y1 - 2014/2
N2 - Lipid and lipoprotein metabolism in zebrafish and in humans are remarkably similar. Zebrafish express all major nuclear receptors, lipid transporters, apolipoproteins and enzymes involved in lipoprotein metabolism. Unlike mice, zebrafish express cetp and the Cetp activity is detected in zebrafish plasma. Feeding zebrafish a high cholesterol diet, without any genetic intervention, results in significant hypercholesterolemia and robust lipoprotein oxidation, making zebrafish an attractive animal model to study mechanisms relevant to early development of human atherosclerosis. These studies are facilitated by the optical transparency of zebrafish larvae and the availability of transgenic zebrafish expressing fluorescent proteins in endothelial cells and macrophages. Thus, vascular processes can be monitored in live animals. In this review article, we discuss recent advances in using dyslipidemic zebrafish in atherosclerosis-related studies. We also summarize recent work connecting lipid metabolism with regulation of angiogenesis, the work that considerably benefited from using the zebrafish model. These studies uncovered the role of aibp, abca1, abcg1, mtp, apoB, and apoC2 in regulation of angiogenesis in zebrafish and paved the way for future studies in mammals, which may suggest new therapeutic approaches to modulation of excessive or diminished angiogenesis contributing to the pathogenesis of human disease.
AB - Lipid and lipoprotein metabolism in zebrafish and in humans are remarkably similar. Zebrafish express all major nuclear receptors, lipid transporters, apolipoproteins and enzymes involved in lipoprotein metabolism. Unlike mice, zebrafish express cetp and the Cetp activity is detected in zebrafish plasma. Feeding zebrafish a high cholesterol diet, without any genetic intervention, results in significant hypercholesterolemia and robust lipoprotein oxidation, making zebrafish an attractive animal model to study mechanisms relevant to early development of human atherosclerosis. These studies are facilitated by the optical transparency of zebrafish larvae and the availability of transgenic zebrafish expressing fluorescent proteins in endothelial cells and macrophages. Thus, vascular processes can be monitored in live animals. In this review article, we discuss recent advances in using dyslipidemic zebrafish in atherosclerosis-related studies. We also summarize recent work connecting lipid metabolism with regulation of angiogenesis, the work that considerably benefited from using the zebrafish model. These studies uncovered the role of aibp, abca1, abcg1, mtp, apoB, and apoC2 in regulation of angiogenesis in zebrafish and paved the way for future studies in mammals, which may suggest new therapeutic approaches to modulation of excessive or diminished angiogenesis contributing to the pathogenesis of human disease.
UR - http://www.scopus.com/inward/record.url?scp=84895076295&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84895076295&partnerID=8YFLogxK
U2 - 10.1016/j.trsl.2013.09.004
DO - 10.1016/j.trsl.2013.09.004
M3 - Review article
C2 - 24095954
AN - SCOPUS:84895076295
SN - 1931-5244
VL - 163
SP - 99
EP - 108
JO - Translational Research
JF - Translational Research
IS - 2
ER -