TY - JOUR
T1 - Xlcaax-1 is localized to the basolateral membrane of kidney tubule and other polarized epithelia during Xenopus development
AU - Cornish, Judith A.
AU - Kloc, Malgorzata
AU - Decker, Glen L.
AU - Reddy, Bramham A.
AU - Etkin, Laurence D.
N1 - Funding Information:
This work was supportedb y Grants DCB-8608690a nd DCB-9007410 from the National ScienceF oundation to L.D.E. and a Rosalie B. Hite Predoctoral Fellowship to J.A.C. We thank Dr. Michael Klymkowsky, University of Colorado at Boulder, for advice concerning the whole-mount immunocytochemistryt echnique.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 1992/3
Y1 - 1992/3
N2 - Xlcaax-1 is a novel, maternally expressed, 110-kDa, CAAX box containing protein that undergoes isoprenylation and palmitoylation through which it associates with the plasma membrane. We report here the cellular and subcellular localization of the xlcaax-1 protein during development of Xenopus laevis. Whole-mount immunocytochemistry and immunoperoxidase staining of tissue sections show that during development the xlcaax-1 protein accumulation is coincident with the differentiation of the epidermis, pronephros, and mesonephros. In the pronephros and mesonephros the xlcaax-1 protein is localized to the basolateral membrane of differentiated tubule epithelial cells. Thus, the xlcaax-1 protein serves as a marker for tubule formation and polarization during Xenopus kidney development. Xlcaax-1 may also be used as a marker for the functional differentiation of the epidermis and the epidermally derived portions of the lens and some cranial nerves. Western blot analysis shows that in the adult the xlcaax-1 protein is most abundant in kidney. Immunogold EM analysis shows that the xlcaax-1 protein is highly enriched in the basal infoldings of the basolateral membrane of the epithelial cells in adult kidney distal tubules. In addition, immunoperoxidase staining of tissue sections detected low levels of xlcaax-1 protein in the epithelial cells of skin, urinary bladder, gall bladder, and parietal glands of the stomach. The localization pattern of xlcaax-1 suggests that the protein may function in association with an ion transport channel or pump.
AB - Xlcaax-1 is a novel, maternally expressed, 110-kDa, CAAX box containing protein that undergoes isoprenylation and palmitoylation through which it associates with the plasma membrane. We report here the cellular and subcellular localization of the xlcaax-1 protein during development of Xenopus laevis. Whole-mount immunocytochemistry and immunoperoxidase staining of tissue sections show that during development the xlcaax-1 protein accumulation is coincident with the differentiation of the epidermis, pronephros, and mesonephros. In the pronephros and mesonephros the xlcaax-1 protein is localized to the basolateral membrane of differentiated tubule epithelial cells. Thus, the xlcaax-1 protein serves as a marker for tubule formation and polarization during Xenopus kidney development. Xlcaax-1 may also be used as a marker for the functional differentiation of the epidermis and the epidermally derived portions of the lens and some cranial nerves. Western blot analysis shows that in the adult the xlcaax-1 protein is most abundant in kidney. Immunogold EM analysis shows that the xlcaax-1 protein is highly enriched in the basal infoldings of the basolateral membrane of the epithelial cells in adult kidney distal tubules. In addition, immunoperoxidase staining of tissue sections detected low levels of xlcaax-1 protein in the epithelial cells of skin, urinary bladder, gall bladder, and parietal glands of the stomach. The localization pattern of xlcaax-1 suggests that the protein may function in association with an ion transport channel or pump.
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U2 - 10.1016/0012-1606(92)90011-5
DO - 10.1016/0012-1606(92)90011-5
M3 - Article
C2 - 1537427
AN - SCOPUS:0026547903
VL - 150
SP - 108
EP - 120
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 1
ER -