TY - JOUR
T1 - Wool Keratin 3D Scaffolds with Light-Triggered Antimicrobial Activity
AU - Ferroni, Claudia
AU - Sotgiu, Giovanna
AU - Sagnella, Anna
AU - Varchi, Greta
AU - Guerrini, Andrea
AU - Giuri, Demetra
AU - Polo, Eleonora
AU - Orlandi, Viviana Teresa
AU - Marras, Emanuela
AU - Gariboldi, Marzia
AU - Monti, Elena
AU - Aluigi, Annalisa
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/9/12
Y1 - 2016/9/12
N2 - Photoactivatable keratin sponges were prepared from protein aqueous solutions by the freeze-drying method, followed by photofunctionalization with two different photosensitizers (PS): Azure A (AzA) and 5,10,15,20-tetrakis [4-(2-N,N,N-trimethylethylthio)-2,3,5,6-tetrafluorophenyl]porphyrin tetraiodide salt (TTFAP). The prepared sponges have a porosity between 49% and 80% and a mean pore size in the 37-80 μm range. As compared to AzA, TTFAP interacts more strongly with the sponges as demonstrated by a lower PS release (6% vs 20%), a decreased swelling ratio (1.6 vs 7.4), and a slower biodegradation rate. Nevertheless, AzA-loaded sponges showed the highest photoactivity, as also demonstrated by their higher antibactericidal activity toward both Gram-positive and Gram-negative bacteria. The obtained results suggest that the antimicrobial photodynamic effect can be finely triggered through a proper selection of the amount and type of photosensitizer, as well as through the irradiation time. Finally, all the prepared sponges support human fibroblast cells growth, while no significant cell viability impairment is observed upon light irradiation.
AB - Photoactivatable keratin sponges were prepared from protein aqueous solutions by the freeze-drying method, followed by photofunctionalization with two different photosensitizers (PS): Azure A (AzA) and 5,10,15,20-tetrakis [4-(2-N,N,N-trimethylethylthio)-2,3,5,6-tetrafluorophenyl]porphyrin tetraiodide salt (TTFAP). The prepared sponges have a porosity between 49% and 80% and a mean pore size in the 37-80 μm range. As compared to AzA, TTFAP interacts more strongly with the sponges as demonstrated by a lower PS release (6% vs 20%), a decreased swelling ratio (1.6 vs 7.4), and a slower biodegradation rate. Nevertheless, AzA-loaded sponges showed the highest photoactivity, as also demonstrated by their higher antibactericidal activity toward both Gram-positive and Gram-negative bacteria. The obtained results suggest that the antimicrobial photodynamic effect can be finely triggered through a proper selection of the amount and type of photosensitizer, as well as through the irradiation time. Finally, all the prepared sponges support human fibroblast cells growth, while no significant cell viability impairment is observed upon light irradiation.
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U2 - 10.1021/acs.biomac.6b00697
DO - 10.1021/acs.biomac.6b00697
M3 - Article
C2 - 27463471
AN - SCOPUS:84987707824
SN - 1525-7797
VL - 17
SP - 2882
EP - 2890
JO - Biomacromolecules
JF - Biomacromolecules
IS - 9
ER -