Wild-type p53 binds to the TATA-binding protein and represses transcription

E. Seto; A. Usheva; G. P. Zambetti; J. Momand; N. Horikoshi; R. Weinmann; A. J. Levine; T. Shenk

doi:10.1073/pnas.89.24.12028

Wild-type p53 binds to the TATA-binding protein and represses transcription

E. Seto, A. Usheva, G. P. Zambetti, J. Momand, N. Horikoshi, R. Weinmann, A. J. Levine, T. Shenk

Academic Institute

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464 Scopus citations

Abstract

p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

Original language	English (US)
Pages (from-to)	12028-12032
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	89
Issue number	24
DOIs	https://doi.org/10.1073/pnas.89.24.12028
State	Published - 1992

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title = "Wild-type p53 binds to the TATA-binding protein and represses transcription",

abstract = "p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.",

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T1 - Wild-type p53 binds to the TATA-binding protein and represses transcription

AU - Seto, E.

AU - Usheva, A.

AU - Zambetti, G. P.

AU - Momand, J.

AU - Horikoshi, N.

AU - Weinmann, R.

AU - Levine, A. J.

AU - Shenk, T.

PY - 1992

Y1 - 1992

N2 - p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

AB - p53 activates transcription of genes with a p53 response element, and it can repress genes lacking the element. Here we demonstrate that wild-type but not mutant p53 inhibits transcription in a HeLa nuclear extract from minimal promoters. Wild-type but not mutant p53 binds to human TATA-binding protein (TBP). p53 does not bind to yeast TBP, and it cannot inhibit transcription in a HeLa extract where yeast TBP substitutes for human TBP. These results suggest a model in which p53 binds to TBP and interferes with transcriptional initiation.

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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ER -

Wild-type p53 binds to the TATA-binding protein and represses transcription

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