Whole-cell repetitive element sequence-based polymerase chain reaction allows rapid assessment of clonal relationships of bacterial isolates

C. R. Woods, James Versalovic, T. Koeuth, J. R. Lupski

Research output: Contribution to journalComment/debatepeer-review

116 Scopus citations

Abstract

Repetitive element sequence-based polymerase chain reaction (rep-PCR) enables the generation of DNA fingerprints which discriminate bacterial species and strains. We describe the application of whole-cell methods which allow specimens from broth cultures or colonies from agar plates to be utilized directly in the PCR mixture. The rep-PCR-generated DNA fingerprints obtained with whole-cell samples match results obtained with genomic DNA templates. Examples with different gram-negative bacteria (e.g., Citrobacter diversus, Escherichia coli, and Pseudomonas aeruginosa) and gram-positive bacteria (e.g., Staphylococcus aureus and Streptococcus pneumoniae) are demonstrated. Rapid specimen preparation methods enable rep-PCR-based fingerprinting to be competed in several hours and, therefore, allows the timely analysis of epidemiological relationships.

Original languageEnglish (US)
Pages (from-to)1927-1931
Number of pages5
JournalJournal of Clinical Microbiology
Volume31
Issue number7
DOIs
StatePublished - 1993

ASJC Scopus subject areas

  • Microbiology (medical)

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