Warm Autoimmune Hemolytic Anemia and Direct Antiglobulin Testing with a False-Negative Result in a 53-Year-Old Man: The DAT Will Set You Free

Michael Losos, Diane Hamad, Sarita Joshi, Scott Scrape, Jian Chen

Research output: Contribution to journalArticle

Abstract

Warm autoimmune hemolytic anemia (WAIHA), the most common of the relatively uncommon autoimmune-mediated hemolytic anemias (AIHAs), is mediated by polyclonal immunoglobulin (Ig)G autoantibodies in most cases. Herein, we present a case of WAIHA involving a direct antiglobulin test (DAT) with an initially negative result. Using a modified DAT protocol, repeat testing of the same specimen material from a previously healthy 53-year-old man yielded positive results. This case demonstrates that investigation of an apparently negative DAT result plays a critical role in the differential diagnosis of patients with rapidly progressing hemolytic anemia and the reversal of that decline.

Original languageEnglish (US)
Pages (from-to)227-232
Number of pages6
JournalLaboratory Medicine
Volume47
Issue number3
DOIs
StatePublished - Aug 1 2016

Keywords

  • DAT negative
  • DAT protocol
  • WAIHA diagnosis
  • WAIHA treatment
  • direct antiglobulin test (DAT)
  • warm autoimmune hemolytic anemia (WAIHA)

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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