Volumetric bar-chart chips for biosensing

Yujun Song, Ying Li, Lidong Qin

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

The volumetric bar-chart chip (V-Chip) is a microfluidics-based, point-of-care (POC) device for the multiplexed and quantitative measurement of biomarkers. Volumetric readouts, based on the measurement of oxygen generated by a reaction between catalase and hydrogen peroxide, allow instant visual quantitation of target biomarkers and provide visualized bar charts without any assistance from instruments and without the need for data processing or graphics plotting. V-Chip shows potential capabilities in POC and personalized diagnostics; for instance, it can be utilized for making high-throughput, multiplexed, and quantitative measurements. Further, this system is highly portable and can be performed at low cost. The development of the V-Chip thus marks a POC milestone and opens up the possibility of instrument-free personalized diagnostics. Here, we describe the protocols for the fabrication of V-Chip and the use of silica nanoparticles as the probe carrier for the V-Chip-based enzyme-linked immunosorbent assay (ELISA) for the detection of biomarkers.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages105-115
Number of pages11
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1570
ISSN (Print)1064-3745

Keywords

  • Biomarkers
  • ELISA
  • Multiplex
  • Point-of-care
  • Quantitation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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