Visualization and identification of IL-7 producing cells in reporter mice

Renata I. Mazzucchelli, Søren Warming, Scott M. Lawrence, Masaru Ishii, Mehrnoosh Abshari, A. Vallance Washington, Lionel Feigenbaum, Andrew C. Warner, Davis J. Sims, Wen Qing Li, Julie A. Hixon, Daniel H.D. Gray, Benjamin E. Rich, Matthew Morrow, Miriam R. Anver, James Cherry, Dieter Naf, Lawrence R. Sternberg, Daniel W. McVicar, Andrew G. FarrRonald N. Germain, Keith Rogers, Nancy A. Jenkins, Neal G. Copeland, Scott K. Durum

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

Interleukin-7 (IL-7) is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial chromosome (BAC) transgenic mouse expressing ECFP in the Il7 locus. The construct lacked a signal peptide and ECFP (enhanced cyan fluorescent protein ) accumulated inside IL-7-producing stromal cells in thoracic thymus, cervical thymus and bone marrow. In thymus, an extensive reticular network of IL-7-containing processes extended from cortical and medullary epithelial cells, closely contacting thymocytes. Central memory CD8 T cells, which require IL-7 and home to bone marrow, physically associated with IL-7-producing cells as we demonstrate by intravital imaging.

Original languageEnglish (US)
Article numbere7637
JournalPLoS ONE
Volume4
Issue number11
DOIs
StatePublished - Nov 10 2009

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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