Visualization and identification of IL-7 producing cells in reporter mice

Renata I. Mazzucchelli; Søren Warming; Scott M. Lawrence; Masaru Ishii; Mehrnoosh Abshari; A. Vallance Washington; Lionel Feigenbaum; Andrew C. Warner; Davis J. Sims; Wen Qing Li; Julie A. Hixon; Daniel H.D. Gray; Benjamin E. Rich; Matthew Morrow; Miriam R. Anver; James Cherry; Dieter Naf; Lawrence R. Sternberg; Daniel W. McVicar; Andrew G. Farr; Ronald N. Germain; Keith Rogers; Nancy A. Jenkins; Neal G. Copeland; Scott K. Durum

doi:10.1371/journal.pone.0007637

Visualization and identification of IL-7 producing cells in reporter mice

Renata I. Mazzucchelli, Søren Warming, Scott M. Lawrence, Masaru Ishii, Mehrnoosh Abshari, A. Vallance Washington, Lionel Feigenbaum, Andrew C. Warner, Davis J. Sims, Wen Qing Li, Julie A. Hixon, Daniel H.D. Gray, Benjamin E. Rich, Matthew Morrow, Miriam R. Anver, James Cherry, Dieter Naf, Lawrence R. Sternberg, Daniel W. McVicar, Andrew G. FarrRonald N. Germain, Keith Rogers, Nancy A. Jenkins, Neal G. Copeland, Scott K. Durum

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Abstract

Interleukin-7 (IL-7) is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial chromosome (BAC) transgenic mouse expressing ECFP in the Il7 locus. The construct lacked a signal peptide and ECFP (enhanced cyan fluorescent protein ) accumulated inside IL-7-producing stromal cells in thoracic thymus, cervical thymus and bone marrow. In thymus, an extensive reticular network of IL-7-containing processes extended from cortical and medullary epithelial cells, closely contacting thymocytes. Central memory CD8 T cells, which require IL-7 and home to bone marrow, physically associated with IL-7-producing cells as we demonstrate by intravital imaging.

Original language	English (US)
Article number	e7637
Journal	PLoS ONE
Volume	4
Issue number	11
DOIs	https://doi.org/10.1371/journal.pone.0007637
State	Published - Nov 10 2009

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Mazzucchelli, R. I., Warming, S., Lawrence, S. M., Ishii, M., Abshari, M., Washington, A. V., Feigenbaum, L., Warner, A. C., Sims, D. J., Li, W. Q., Hixon, J. A., Gray, D. H. D., Rich, B. E., Morrow, M., Anver, M. R., Cherry, J., Naf, D., Sternberg, L. R., McVicar, D. W., ... Durum, S. K. (2009). Visualization and identification of IL-7 producing cells in reporter mice. PLoS ONE, 4(11), Article e7637. https://doi.org/10.1371/journal.pone.0007637

Mazzucchelli, RI, Warming, S, Lawrence, SM, Ishii, M, Abshari, M, Washington, AV, Feigenbaum, L, Warner, AC, Sims, DJ, Li, WQ, Hixon, JA, Gray, DHD, Rich, BE, Morrow, M, Anver, MR, Cherry, J, Naf, D, Sternberg, LR, McVicar, DW, Farr, AG, Germain, RN, Rogers, K, Jenkins, NA , Copeland, NG & Durum, SK 2009, 'Visualization and identification of IL-7 producing cells in reporter mice', PLoS ONE, vol. 4, no. 11, e7637. https://doi.org/10.1371/journal.pone.0007637

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title = "Visualization and identification of IL-7 producing cells in reporter mice",

abstract = "Interleukin-7 (IL-7) is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial chromosome (BAC) transgenic mouse expressing ECFP in the Il7 locus. The construct lacked a signal peptide and ECFP (enhanced cyan fluorescent protein ) accumulated inside IL-7-producing stromal cells in thoracic thymus, cervical thymus and bone marrow. In thymus, an extensive reticular network of IL-7-containing processes extended from cortical and medullary epithelial cells, closely contacting thymocytes. Central memory CD8 T cells, which require IL-7 and home to bone marrow, physically associated with IL-7-producing cells as we demonstrate by intravital imaging.",

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AU - Abshari, Mehrnoosh

AU - Washington, A. Vallance

AU - Feigenbaum, Lionel

AU - Warner, Andrew C.

AU - Sims, Davis J.

AU - Li, Wen Qing

AU - Hixon, Julie A.

AU - Gray, Daniel H.D.

AU - Rich, Benjamin E.

AU - Morrow, Matthew

AU - Anver, Miriam R.

AU - Cherry, James

AU - Naf, Dieter

AU - Sternberg, Lawrence R.

AU - McVicar, Daniel W.

AU - Farr, Andrew G.

AU - Germain, Ronald N.

AU - Rogers, Keith

AU - Jenkins, Nancy A.

AU - Copeland, Neal G.

AU - Durum, Scott K.

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Visualization and identification of IL-7 producing cells in reporter mice

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