Virus detection using nanoelectromechanical devices

B. Ilic; Y. Yang; H. G. Craighead

doi:10.1063/1.1794378

Virus detection using nanoelectromechanical devices

B. Ilic, Y. Yang, H. G. Craighead

Houston Methodist

Research output: Contribution to journal › Article › peer-review

360 Scopus citations

Abstract

The binding events of the baculovirus was measured using arrays of chemically functionalized, surface micromachined polycrystalline silicon cantilevers. The 0.5 μm×6 μm cantilevers have mass sensitivities on the order of 10 ^-19 g/Hz, that enables to detect an immobilized AcV1 antibody monolayer corresponsing to mass of about 3×10 ^-15 g. The resonant frequency shift was observed from the adsorbed mass of the virus particles distinguished solutions of virus concentrations and varies between 10 ⁵ and 10 ⁷ pfu/ml. The mass of single-virus particles bound to the cantilever can also be detected using cantilevers.

Original language	English (US)
Pages (from-to)	2604-2606
Number of pages	3
Journal	Applied Physics Letters
Volume	85
Issue number	13
DOIs	https://doi.org/10.1063/1.1794378
State	Published - Sep 27 2004

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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10.1063/1.1794378

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@article{2a4220e9023c4f4b909187bdf8d3fd97,

title = "Virus detection using nanoelectromechanical devices",

abstract = "The binding events of the baculovirus was measured using arrays of chemically functionalized, surface micromachined polycrystalline silicon cantilevers. The 0.5 μm×6 μm cantilevers have mass sensitivities on the order of 10 -19 g/Hz, that enables to detect an immobilized AcV1 antibody monolayer corresponsing to mass of about 3×10 -15 g. The resonant frequency shift was observed from the adsorbed mass of the virus particles distinguished solutions of virus concentrations and varies between 10 5 and 10 7 pfu/ml. The mass of single-virus particles bound to the cantilever can also be detected using cantilevers.",

author = "B. Ilic and Y. Yang and Craighead, \{H. G.\}",

note = "Funding Information: The authors would like to thank Professor Michael Shuler (Department of Chemical and Biomolecular Engineering, Cornell University) for providing facilities for insect cell culture and virus amplification. Additionally, hybridoma cell line producing AcV1 monoclonal antibody against the gp64 coat protein of Autographa californica nuclear polyhedrosis virus (AcNPV) was generously provided by Professor Gary Blissard (Boyce Thompson Institute for Plant Research, Ithaca, NY). Furthermore, the authors would like to thank the staff at the Cornell NanoScale Facility for helpful discussion and generous aid in fabrication. This work was supported in part by DARPA, NSF through the Nanobiotechnology Center, and New York State.",

year = "2004",

month = sep,

day = "27",

doi = "10.1063/1.1794378",

language = "English (US)",

volume = "85",

pages = "2604--2606",

journal = "Applied Physics Letters",

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T1 - Virus detection using nanoelectromechanical devices

AU - Ilic, B.

AU - Yang, Y.

AU - Craighead, H. G.

N1 - Funding Information: The authors would like to thank Professor Michael Shuler (Department of Chemical and Biomolecular Engineering, Cornell University) for providing facilities for insect cell culture and virus amplification. Additionally, hybridoma cell line producing AcV1 monoclonal antibody against the gp64 coat protein of Autographa californica nuclear polyhedrosis virus (AcNPV) was generously provided by Professor Gary Blissard (Boyce Thompson Institute for Plant Research, Ithaca, NY). Furthermore, the authors would like to thank the staff at the Cornell NanoScale Facility for helpful discussion and generous aid in fabrication. This work was supported in part by DARPA, NSF through the Nanobiotechnology Center, and New York State.

PY - 2004/9/27

Y1 - 2004/9/27

N2 - The binding events of the baculovirus was measured using arrays of chemically functionalized, surface micromachined polycrystalline silicon cantilevers. The 0.5 μm×6 μm cantilevers have mass sensitivities on the order of 10 -19 g/Hz, that enables to detect an immobilized AcV1 antibody monolayer corresponsing to mass of about 3×10 -15 g. The resonant frequency shift was observed from the adsorbed mass of the virus particles distinguished solutions of virus concentrations and varies between 10 5 and 10 7 pfu/ml. The mass of single-virus particles bound to the cantilever can also be detected using cantilevers.

AB - The binding events of the baculovirus was measured using arrays of chemically functionalized, surface micromachined polycrystalline silicon cantilevers. The 0.5 μm×6 μm cantilevers have mass sensitivities on the order of 10 -19 g/Hz, that enables to detect an immobilized AcV1 antibody monolayer corresponsing to mass of about 3×10 -15 g. The resonant frequency shift was observed from the adsorbed mass of the virus particles distinguished solutions of virus concentrations and varies between 10 5 and 10 7 pfu/ml. The mass of single-virus particles bound to the cantilever can also be detected using cantilevers.

UR - https://www.scopus.com/pages/publications/7544230375

UR - https://www.scopus.com/inward/citedby.url?scp=7544230375&partnerID=8YFLogxK

U2 - 10.1063/1.1794378

DO - 10.1063/1.1794378

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VL - 85

SP - 2604

EP - 2606

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 13

ER -

Virus detection using nanoelectromechanical devices

Abstract

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