Vector competence analyses on aedes aegypti mosquitoes using zika virus

Sasha R. Azar; Scott C. Weaver

doi:10.3791/61112

Vector competence analyses on aedes aegypti mosquitoes using zika virus

Sasha R. Azar, Scott C. Weaver

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The procedures presented describe a generalized methodology to infect Aedes aegypti mosquitoes with Zika virus under laboratory conditions to determine the rate of infection, disseminated infection, and potential transmission of the virus in the mosquito population in question. These procedures are widely utilized with various modifications in vector competence evaluations globally. They are important in determining the potential role that a given mosquito (i.e., species, population, individual) may play in the transmission of a given agent.

Original language	English (US)
Article number	e61112
Pages (from-to)	1-7
Number of pages	7
Journal	Journal of Visualized Experiments
Volume	2020
Issue number	159
DOIs	https://doi.org/10.3791/61112
State	Published - May 2020

Keywords

Aedes
Aedes aegypti
Arbovirus
Immunology and Infection
Issue 159
Mosquito
Vector competence
Zika

ASJC Scopus subject areas

General Neuroscience
General Chemical Engineering
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.3791/61112

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title = "Vector competence analyses on aedes aegypti mosquitoes using zika virus",

abstract = "The procedures presented describe a generalized methodology to infect Aedes aegypti mosquitoes with Zika virus under laboratory conditions to determine the rate of infection, disseminated infection, and potential transmission of the virus in the mosquito population in question. These procedures are widely utilized with various modifications in vector competence evaluations globally. They are important in determining the potential role that a given mosquito (i.e., species, population, individual) may play in the transmission of a given agent.",

keywords = "Aedes, Aedes aegypti, Arbovirus, Immunology and Infection, Issue 159, Mosquito, Vector competence, Zika",

author = "Azar, {Sasha R.} and Weaver, {Scott C.}",

note = "Funding Information: We acknowledge the staff of the World Reference Center for Emerging Viruses and Arboviruses (WRCEVA): Dr. Robert Tesh, Hilda Guzman, Dr. Kenneth Plante, Dr. Jessica Plante, Dionna Scharton, and Divya Mirchandani, for their tireless work in curating and providing many of the viral strains used for our and other groups{\textquoteright} vector competence experiments. The presented work was funded by the McLaughlin Fellowship Fund (SRA) and NIH grants AI120942 and AI121452. Publisher Copyright: {\textcopyright} 2020, Journal of Visualized Experiments. All rights reserved.",

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doi = "10.3791/61112",

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N2 - The procedures presented describe a generalized methodology to infect Aedes aegypti mosquitoes with Zika virus under laboratory conditions to determine the rate of infection, disseminated infection, and potential transmission of the virus in the mosquito population in question. These procedures are widely utilized with various modifications in vector competence evaluations globally. They are important in determining the potential role that a given mosquito (i.e., species, population, individual) may play in the transmission of a given agent.

AB - The procedures presented describe a generalized methodology to infect Aedes aegypti mosquitoes with Zika virus under laboratory conditions to determine the rate of infection, disseminated infection, and potential transmission of the virus in the mosquito population in question. These procedures are widely utilized with various modifications in vector competence evaluations globally. They are important in determining the potential role that a given mosquito (i.e., species, population, individual) may play in the transmission of a given agent.

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KW - Vector competence

KW - Zika

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Vector competence analyses on aedes aegypti mosquitoes using zika virus

Abstract

Keywords

ASJC Scopus subject areas

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