Vasodilation by in vivo activation of astrocyte endfeet via two-photon calcium uncaging as a strategy to prevent brain ischemia

Yuanxin Chen, James Mancuso, Zhen Zhao, Xuping Li, Jie Cheng, Gustavo Roman, Stephen T.C. Wong

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Decreased cerebral blood flow causes brain ischemia and plays an important role in the pathophysiology of many neurodegenerative diseases, including Alzheimer's disease and vascular dementia. In this study, we photomodulated astrocytes in the live animal by a combination of two-photon calcium uncaging in the astrocyte endfoot and in vivo imaging of neurovasculature and astrocytes by intravital two-photon microscopy after labeling with cell type specific fluorescent dyes. Our study demonstrates that photomodulation at the endfoot of a single astrocyte led to a 25% increase in the diameter of a neighboring arteriole, which is a crucial factor regulating cerebral microcirculation in downstream capillaries. Two-photon uncaging in the astrocyte soma or endfoot near veins does not show the same effect on microcirculation. These experimental results suggest that infrared photomodulation on astrocyte endfeet may be a strategy to increase cerebral local microcirculation and thus prevent brain ischemia.

Original languageEnglish (US)
Article number126012
JournalJournal of Biomedical Optics
Volume18
Issue number12
DOIs
StatePublished - Dec 2013

Keywords

  • Astrocytes-vasculature
  • Calcium uncaging
  • Cerebral microcirculation
  • Infrared femtosecond laser
  • Intravital two-photon microscopy

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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