Vascular tube formation and angiogenesis induced by polyvinylpyrrolidone-coated silver nanoparticles

Kyeongah Kang, Dae Hyoun Lim, In Hong Choi, Taegyeong Kang, Kangtaek Lee, Eun Yi Moon, Young Yang, Myeong Sok Lee, Jong Seok Lim

Research output: Contribution to journalArticle

61 Scopus citations


Silver nanoparticles (AgNPs) are one of the most commonly used nanomaterials due to their antibacterial properties. In this study, we examined the effects of polyvinylpyrrolidone (PVP)-coated AgNPs (average size 2.3. nm) on angiogenesis in both an in vivo model and an in vitro endothelial cell line, SVEC4-10. Increased angiogenesis was detected around the injection site of AgNP-containing Matrigel in vivo. AgNPs also increased the infiltration of endothelial cells and the hemoglobin (Hb) content in AgNP-Matrigel plugs implanted into mice. AgNPs induced endothelial cell tube formation on growth factor-reduced Matrigel, production of reactive oxygen species (ROS), and production of angiogenic factors, such as vascular endothelial growth factor (VEGF) and nitric oxide (NO), in SVEC4-10 cells. In addition, AgNPs promoted the activation of FAK, Akt, ERK1/2, and p38, which are all involved in VEGF receptor (VEGFR)-mediated signaling. Finally, AgNP-treated tumors caused angiogenesis around tumors in B16F10 melanomas after they were injected into mice, and the Hb concentration in the tumors increased in a concentration-dependent manner with AgNP treatment. Thus, our study suggests that exposure to AgNPs can cause angiogenesis through the production of angiogenic factors.

Original languageEnglish (US)
Pages (from-to)227-234
Number of pages8
JournalToxicology Letters
Issue number3
StatePublished - Sep 10 2011


  • Angiogenesis
  • Matrigel plug
  • Silver nanoparticles
  • VEGF signaling
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Toxicology

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