Vascular tube formation and angiogenesis induced by polyvinylpyrrolidone-coated silver nanoparticles

Kyeongah Kang, Dae Hyoun Lim, In Hong Choi, Taegyeong Kang, Kangtaek Lee, Eun Yi Moon, Young Yang, Myeong Sok Lee, Jong Seok Lim

Research output: Contribution to journalArticlepeer-review

69 Scopus citations


Silver nanoparticles (AgNPs) are one of the most commonly used nanomaterials due to their antibacterial properties. In this study, we examined the effects of polyvinylpyrrolidone (PVP)-coated AgNPs (average size 2.3. nm) on angiogenesis in both an in vivo model and an in vitro endothelial cell line, SVEC4-10. Increased angiogenesis was detected around the injection site of AgNP-containing Matrigel in vivo. AgNPs also increased the infiltration of endothelial cells and the hemoglobin (Hb) content in AgNP-Matrigel plugs implanted into mice. AgNPs induced endothelial cell tube formation on growth factor-reduced Matrigel, production of reactive oxygen species (ROS), and production of angiogenic factors, such as vascular endothelial growth factor (VEGF) and nitric oxide (NO), in SVEC4-10 cells. In addition, AgNPs promoted the activation of FAK, Akt, ERK1/2, and p38, which are all involved in VEGF receptor (VEGFR)-mediated signaling. Finally, AgNP-treated tumors caused angiogenesis around tumors in B16F10 melanomas after they were injected into mice, and the Hb concentration in the tumors increased in a concentration-dependent manner with AgNP treatment. Thus, our study suggests that exposure to AgNPs can cause angiogenesis through the production of angiogenic factors.

Original languageEnglish (US)
Pages (from-to)227-234
Number of pages8
JournalToxicology Letters
Issue number3
StatePublished - Sep 10 2011


  • Angiogenesis
  • Matrigel plug
  • Silver nanoparticles
  • VEGF signaling
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Toxicology


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