Variation of fluorescence in tissue with temperature

Raiyan T. Zaman, Narasimhan Rajaram, Alex Walsh, Jeffrey Oliver, Henry G. Rylander, James W. Tunnell, Ashley J. Welch, Anita Mahadevan-Jansen

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Background: and Objective Previous studies demonstrated a decrease in fluorescence intensity as tissue temperature increased. In vitro samples were increased from room temperature and in vivo canine liver from body temperature. This study investigated variations in fluorescence intensity with temperatures starting at 14°C and compared in vivo and in vitro results for consistency. Study Design/Material and Methods A fiber optic-based noninvasive system was used to characterize the temperature effect on tissue fluorescence in hamster dorsal skin in vivo, and in sclera and cornea of enucleated pig eyes in vitro. As tissue was allowed to progress through the temperature range of 14-42°C, the spectra of auto-fluorescence with respect to temperature was sampled every 1-2 minutes. A pulsed nitrogen laser was used to excite fluorescence through a fiber optic probe with a source-detector aperture separation of 370 Aμm. Results Fluorescence intensity decreased as temperature increased from 14 to 42°C in a phantom containing Rhodamine B dye. Results from both in vivo and in vitro tissue followed the same trend of decreasing intensity as tissue temperature increased from 14°C. Spectral intensity lineshape changed around 450 nm due to absorption from tissue. Conclusion Cooling a tissue increased fluorescence intensity of skin in vivo, in all experiments. In vitro results were consistent with in vivo measurements.

Original languageEnglish (US)
Pages (from-to)36-42
Number of pages7
JournalLasers in Surgery and Medicine
Volume43
Issue number1
DOIs
StatePublished - Jan 2011

Keywords

  • collagen
  • emission spectrum
  • fluorescence intensity
  • fluorescence spectrometer system
  • fluorophores
  • NADH
  • pulsed nitrogen laser

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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