Validation of the MycoAlign system for Mycobacterium spp. identification

Randall J. Olsen, Patricia A. Cernoch, Celest M. Austin, Edward A. Graviss, Daniel H. Farkas, Geoffrey A. Land

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Validation of the MycoAlign assay, a newly developed Mycobacterium spp. identification system based on internal transcribed spacer-1 sequencing, was performed using 50 acid-fast bacilli (AFB)-positive clinical laboratory specimens. Forty-three (86%) diagnostic-level results were obtained, including 38 Mycobacterium spp. and 5 other AFB-positive genera. Three isolates (6%) had suboptimal identity scores with high probability (81-87% identity score). Four (8%) mixed-pattern results were obtained. Forty-five (90%) observations were concordant with the species identification by standard methods, including all controls.

Original languageEnglish (US)
Pages (from-to)105-108
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume59
Issue number1
DOIs
StatePublished - Sep 2007

Keywords

  • 16S
  • hsp65
  • Internal transcribed region
  • ITS-1
  • Molecular diagnostics
  • MycoAlign
  • Mycobacteria
  • PCR
  • Sequencing

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Virology
  • Parasitology
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology

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