Validation of the MycoAlign system for Mycobacterium spp. identification

Randall J. Olsen; Patricia A. Cernoch; Celest M. Austin; Edward A. Graviss; Daniel H. Farkas; Geoffrey A. Land

doi:10.1016/j.diagmicrobio.2007.04.012

Validation of the MycoAlign system for Mycobacterium spp. identification

Randall J. Olsen, Patricia A. Cernoch, Celest M. Austin, Edward A. Graviss, Daniel H. Farkas, Geoffrey A. Land

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Validation of the MycoAlign assay, a newly developed Mycobacterium spp. identification system based on internal transcribed spacer-1 sequencing, was performed using 50 acid-fast bacilli (AFB)-positive clinical laboratory specimens. Forty-three (86%) diagnostic-level results were obtained, including 38 Mycobacterium spp. and 5 other AFB-positive genera. Three isolates (6%) had suboptimal identity scores with high probability (81-87% identity score). Four (8%) mixed-pattern results were obtained. Forty-five (90%) observations were concordant with the species identification by standard methods, including all controls.

Original language	English (US)
Pages (from-to)	105-108
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	59
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2007.04.012
State	Published - Sep 2007

Keywords

16S
hsp65
Internal transcribed region
ITS-1
Molecular diagnostics
MycoAlign
Mycobacteria
PCR
Sequencing

ASJC Scopus subject areas

Infectious Diseases
Immunology and Allergy
Virology
Parasitology
Microbiology
Immunology
Applied Microbiology and Biotechnology

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10.1016/j.diagmicrobio.2007.04.012

Cite this

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title = "Validation of the MycoAlign system for Mycobacterium spp. identification",

abstract = "Validation of the MycoAlign assay, a newly developed Mycobacterium spp. identification system based on internal transcribed spacer-1 sequencing, was performed using 50 acid-fast bacilli (AFB)-positive clinical laboratory specimens. Forty-three (86%) diagnostic-level results were obtained, including 38 Mycobacterium spp. and 5 other AFB-positive genera. Three isolates (6%) had suboptimal identity scores with high probability (81-87% identity score). Four (8%) mixed-pattern results were obtained. Forty-five (90%) observations were concordant with the species identification by standard methods, including all controls.",

keywords = "16S, hsp65, Internal transcribed region, ITS-1, Molecular diagnostics, MycoAlign, Mycobacteria, PCR, Sequencing",

author = "Olsen, {Randall J.} and Cernoch, {Patricia A.} and Austin, {Celest M.} and Graviss, {Edward A.} and Farkas, {Daniel H.} and Land, {Geoffrey A.}",

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journal = "Diagnostic Microbiology and Infectious Disease",

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T1 - Validation of the MycoAlign system for Mycobacterium spp. identification

AU - Olsen, Randall J.

AU - Cernoch, Patricia A.

AU - Austin, Celest M.

AU - Graviss, Edward A.

AU - Farkas, Daniel H.

AU - Land, Geoffrey A.

PY - 2007/9

Y1 - 2007/9

N2 - Validation of the MycoAlign assay, a newly developed Mycobacterium spp. identification system based on internal transcribed spacer-1 sequencing, was performed using 50 acid-fast bacilli (AFB)-positive clinical laboratory specimens. Forty-three (86%) diagnostic-level results were obtained, including 38 Mycobacterium spp. and 5 other AFB-positive genera. Three isolates (6%) had suboptimal identity scores with high probability (81-87% identity score). Four (8%) mixed-pattern results were obtained. Forty-five (90%) observations were concordant with the species identification by standard methods, including all controls.

AB - Validation of the MycoAlign assay, a newly developed Mycobacterium spp. identification system based on internal transcribed spacer-1 sequencing, was performed using 50 acid-fast bacilli (AFB)-positive clinical laboratory specimens. Forty-three (86%) diagnostic-level results were obtained, including 38 Mycobacterium spp. and 5 other AFB-positive genera. Three isolates (6%) had suboptimal identity scores with high probability (81-87% identity score). Four (8%) mixed-pattern results were obtained. Forty-five (90%) observations were concordant with the species identification by standard methods, including all controls.

KW - 16S

KW - hsp65

KW - Internal transcribed region

KW - ITS-1

KW - Molecular diagnostics

KW - MycoAlign

KW - Mycobacteria

KW - PCR

KW - Sequencing

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JO - Diagnostic Microbiology and Infectious Disease

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Validation of the MycoAlign system for Mycobacterium spp. identification

Abstract

Keywords

ASJC Scopus subject areas

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