Validation of dynamic contrast-enhanced magnetic resonance imaging-derived vascular permeability measurements using quantitative autoradiography in the RG2 rat brain tumor model

Moira C. Ferrier, Hemant Sarin, Steve H. Fung, Bawarjan Schatlo, Ryszard M. Pluta, Sandeep N. Gupta, Peter L. Choykeb, Edward H. Oldfield, David Thomasson, John A. Butman

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) is widely used to evaluate tumor permeability, yet measurements have not been directly validated in brain tumors. Our purpose was to compare estimates of forward leakage Ktrans derived from DCE-MRI to the estimates K obtained using [14C] aminoisobutyric acid quantitative autoradiography ([ 14C]AIB QAR), an established method of evaluating blood-tumor barrier permeability. Both DCE-MRI and [14C]AIB QAR were performed in five rats 9 to 11 days following tumor implantation. Ktrans in the tumor was estimated from DCE-MRI using the three-parameter general kinetic model and a measured vascular input function. Ki was estimated from QAR data using regions of interest (ROI) closely corresponding to those used to estimate Ktrans. Ktrans and Ki correlated with each other for two independent sets of central tumor ROI (R = 0.905, P = .035; R = 0.933, P = .021). In an additional six rats, Ktrans was estimated on two occasions to show reproducibility (intraclass coefficient = 0.9993; coefficient of variance = 6.07%). In vivo blood-tumor permeability parameters derived from DCE-MRI are reproducible and correlate with the gold standard for quantifying blood tumor barrier permeability, [14C]AIB QAR.

Original languageEnglish (US)
Pages (from-to)546-555
Number of pages10
JournalNeoplasia
Volume9
Issue number7
DOIs
StatePublished - Jul 2007

Keywords

  • Anti-angiogenesis
  • Blood brain barrier
  • Tumor permeability

ASJC Scopus subject areas

  • Cancer Research

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