Validation of bioassays for assessing the contamination of marine environments

Susanne J. Mcdonald; Kristine L. Willett; Stephen H. Safe; Karla B. Beatty; Michael Steinberg; Philippe Mayor; Mahlon C. Kennicutt

doi:10.1080/10406639608544649

Validation of bioassays for assessing the contamination of marine environments

Susanne J. Mcdonald, Kristine L. Willett, Stephen H. Safe, Karla B. Beatty, Michael Steinberg, Philippe Mayor, Mahlon C. Kennicutt

Research output: Contribution to journal › Article

3 Scopus citations

Abstract

Various biomarkers were used to determine the exposure offish (Arius felis and Micropogon undulatus) from Galveston Bay (GB), Texas, USA to organic contaminants. Sediment levels of polynuclear aromatic hydrocarbons (PAHs) in GB ranged from 81 to >1000 ng/g and polychlorinated biphenyls (PCBs) were <20 ng/g at all stations. No significant differences in hepatic concentrations of contaminants and ethoxyresorufin O-deethylase (EROD) activity, CYP1A mRNA levels, and DNA adducts were found in A. felis from GB. However, significant differences in biliary concentrations of naphthalene, phenanthrene, and benzo[a]pyrene metabolites were observed. Induced EROD activities and elevated levels of biliary PAH metabolites were measured in M. undulatus from the two most contaminated sites in GB. Induction toxic equivalents (I-TEQs), derived from dosing rat hepatoma H4IIE cells with hepatic extracts of A. felis, were correlated with tissue levels of ≥4-ring PAHs.

Original language	English (US)
Pages (from-to)	57-65
Number of pages	9
Journal	Polycyclic Aromatic Compounds
Volume	11
Issue number	1-4
DOIs	https://doi.org/10.1080/10406639608544649
State	Published - 1996

Keywords

Biomarkers
Fish
Marine
Organic contaminants

ASJC Scopus subject areas

Polymers and Plastics
Organic Chemistry
Materials Chemistry

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10.1080/10406639608544649

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title = "Validation of bioassays for assessing the contamination of marine environments",

abstract = "Various biomarkers were used to determine the exposure offish (Arius felis and Micropogon undulatus) from Galveston Bay (GB), Texas, USA to organic contaminants. Sediment levels of polynuclear aromatic hydrocarbons (PAHs) in GB ranged from 81 to >1000 ng/g and polychlorinated biphenyls (PCBs) were <20 ng/g at all stations. No significant differences in hepatic concentrations of contaminants and ethoxyresorufin O-deethylase (EROD) activity, CYP1A mRNA levels, and DNA adducts were found in A. felis from GB. However, significant differences in biliary concentrations of naphthalene, phenanthrene, and benzo[a]pyrene metabolites were observed. Induced EROD activities and elevated levels of biliary PAH metabolites were measured in M. undulatus from the two most contaminated sites in GB. Induction toxic equivalents (I-TEQs), derived from dosing rat hepatoma H4IIE cells with hepatic extracts of A. felis, were correlated with tissue levels of ≥4-ring PAHs.",

keywords = "Biomarkers, Fish, Marine, Organic contaminants",

author = "Mcdonald, {Susanne J.} and Willett, {Kristine L.} and Safe, {Stephen H.} and Beatty, {Karla B.} and Michael Steinberg and Philippe Mayor and Kennicutt, {Mahlon C.}",

year = "1996",

doi = "10.1080/10406639608544649",

language = "English (US)",

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pages = "57--65",

journal = "Polycyclic Aromatic Compounds",

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T1 - Validation of bioassays for assessing the contamination of marine environments

AU - Mcdonald, Susanne J.

AU - Willett, Kristine L.

AU - Safe, Stephen H.

AU - Beatty, Karla B.

AU - Steinberg, Michael

AU - Mayor, Philippe

AU - Kennicutt, Mahlon C.

PY - 1996

Y1 - 1996

N2 - Various biomarkers were used to determine the exposure offish (Arius felis and Micropogon undulatus) from Galveston Bay (GB), Texas, USA to organic contaminants. Sediment levels of polynuclear aromatic hydrocarbons (PAHs) in GB ranged from 81 to >1000 ng/g and polychlorinated biphenyls (PCBs) were <20 ng/g at all stations. No significant differences in hepatic concentrations of contaminants and ethoxyresorufin O-deethylase (EROD) activity, CYP1A mRNA levels, and DNA adducts were found in A. felis from GB. However, significant differences in biliary concentrations of naphthalene, phenanthrene, and benzo[a]pyrene metabolites were observed. Induced EROD activities and elevated levels of biliary PAH metabolites were measured in M. undulatus from the two most contaminated sites in GB. Induction toxic equivalents (I-TEQs), derived from dosing rat hepatoma H4IIE cells with hepatic extracts of A. felis, were correlated with tissue levels of ≥4-ring PAHs.

AB - Various biomarkers were used to determine the exposure offish (Arius felis and Micropogon undulatus) from Galveston Bay (GB), Texas, USA to organic contaminants. Sediment levels of polynuclear aromatic hydrocarbons (PAHs) in GB ranged from 81 to >1000 ng/g and polychlorinated biphenyls (PCBs) were <20 ng/g at all stations. No significant differences in hepatic concentrations of contaminants and ethoxyresorufin O-deethylase (EROD) activity, CYP1A mRNA levels, and DNA adducts were found in A. felis from GB. However, significant differences in biliary concentrations of naphthalene, phenanthrene, and benzo[a]pyrene metabolites were observed. Induced EROD activities and elevated levels of biliary PAH metabolites were measured in M. undulatus from the two most contaminated sites in GB. Induction toxic equivalents (I-TEQs), derived from dosing rat hepatoma H4IIE cells with hepatic extracts of A. felis, were correlated with tissue levels of ≥4-ring PAHs.

KW - Biomarkers

KW - Fish

KW - Marine

KW - Organic contaminants

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