TY - JOUR
T1 - Utilization of cultured chick embryo hepatocytes as in vitro bioassays for polychlorinated biphenyls (PCBs)
T2 - Quantitative structure-induction relationships
AU - Yao, C.
AU - Panigrahy, B.
AU - Safe, S.
N1 - Funding Information:
ACKNOWLEDGEMENTS The financial assistance of the National Institutes of Health (P42-ESO4917) and the Texas Agricultural Experiment Station are gratefully acknowledged. S. Safe is a Burroughs Wellcome Toxicology Scholar.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1990
Y1 - 1990
N2 - The concentration-dependent induction of aryl hydrocarbon hydroxylase (AHH) and ethoxyresorufin-O-deethylase (EROD) activities by several PCB congeners was determined in primary chick embryo hepatocytes in culture. The most active inducers were the coplanar PCBs, 3,3′,4,4′-tetra- and 3,3′,4,4′5-pentachlorobiphenyl (pentaCB) with EC50 values of 1.2 × 10-9 and 1.5 × 10-9 M (AHH induction) and 2.3 × 10-9 and 1.2 × 10-9 M (EROD induction) respectively. The corresponding EC50 values for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) were 2.4 × 10-11 and 7.2 × 10-11 M for AHH and EROD induction respectively. Three monoortho coplanar PCB analogs, 2,3,3′,4,4′-pentaCB, 2,3′,4,4′,5-pentaCB and 2,3,3′,4,4′,5-hexaCB also exhibited induction activity but their corresponding EC50 values were much higher than observed for the coplanar PCBs. Several di- and triortho-substituted PCB congeners were weakly active as inducers of AHH and EROD activity. The structure-induction relationships for the coplanar and monoortho coplanar PCBs in the chick embryo hepatocytes were comparable to those previously reported in rat hepatoma H-4-II E cells and in in vivo studies.
AB - The concentration-dependent induction of aryl hydrocarbon hydroxylase (AHH) and ethoxyresorufin-O-deethylase (EROD) activities by several PCB congeners was determined in primary chick embryo hepatocytes in culture. The most active inducers were the coplanar PCBs, 3,3′,4,4′-tetra- and 3,3′,4,4′5-pentachlorobiphenyl (pentaCB) with EC50 values of 1.2 × 10-9 and 1.5 × 10-9 M (AHH induction) and 2.3 × 10-9 and 1.2 × 10-9 M (EROD induction) respectively. The corresponding EC50 values for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) were 2.4 × 10-11 and 7.2 × 10-11 M for AHH and EROD induction respectively. Three monoortho coplanar PCB analogs, 2,3,3′,4,4′-pentaCB, 2,3′,4,4′,5-pentaCB and 2,3,3′,4,4′,5-hexaCB also exhibited induction activity but their corresponding EC50 values were much higher than observed for the coplanar PCBs. Several di- and triortho-substituted PCB congeners were weakly active as inducers of AHH and EROD activity. The structure-induction relationships for the coplanar and monoortho coplanar PCBs in the chick embryo hepatocytes were comparable to those previously reported in rat hepatoma H-4-II E cells and in in vivo studies.
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U2 - 10.1016/0045-6535(90)90123-B
DO - 10.1016/0045-6535(90)90123-B
M3 - Article
AN - SCOPUS:0025651660
VL - 21
SP - 1007
EP - 1016
JO - Chemosphere
JF - Chemosphere
SN - 0045-6535
IS - 8
ER -