Use of [3H]-GBR12935 to measure dopaminergic nerve terminal growth into the developing rat striatum

Weidong Le, J. Robert Bostwick, Stanley H. Appel

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

In this study we determined the temporal association between the appearance of the dopamine transporter, measured by 1-[2-(diphenylmethoxy)ethyl)]-4-(3-phenylpropyl)-piperazine ([3H]-GBR12935), a potent and selective inhibitor of dopamine uptake, and other biochemical markers of dopaminergic nerve terminal growth into the developing striatum. [3H]-GBR12935 binding was minimally detected in the rudimentary striatum of embryonic day 14 rat brains, increased to 23% of the adult level by birth, and reached the adult level during the fifth postnatal week. This finding contrasts with a slower developmental increase in [3H]-dopamine uptake, a functional measure of the transporter. Tyrosine hydroxylase activity levels followed a developmental curve similar to that of [3H]-GBR12935 binding but did not reach adult levels until the 7th postnatal week. Dopamine content increased at a slower rate, being only 10% and 92% of the adult level at birth and postnatal week 8, respectively. These results indicate that the appearance of a structural, but not optimally functional, dopamine transporter may be the earliest detectable biochemical index of dopaminergic nerve terminal growth into the striatum during development.

Original languageEnglish (US)
Pages (from-to)375-377
Number of pages3
JournalDevelopmental Brain Research
Volume67
Issue number2
DOIs
StatePublished - Jun 19 1992

Keywords

  • Development
  • Dopamine
  • Dopamine uptake
  • GBR12935
  • Striatum
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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