Uroplakin II is a more sensitive immunohistochemical marker than uroplakin III in urothelial carcinoma and its variants

Wenping Li, Yu Liang, Michael T. Deavers, Ashish M. Kamat, Surena F. Matin, Colin P. Dinney, Bogdan Czerniak, Charles C. Guo

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Objectives: Uroplakin (UP) II and UPIII are highly specific immunohistochemical markers for urothelial differentiation. Here we studied the sensitivity of UPII and UPIII in conventional and variant urothelial carcinomas (UCs). Methods: Immunohistochemical staining for UPII and UPIII was performed on tissue microarray slides, including 105 conventional bladder UCs (BUCs), 90 upper urinary tract UCs (UUTUCs), and 47 micropapillary, 16 plasmacytoid, 22 small cell carcinoma, and 41 sarcomatoid UC variants. Results: UPII expression was significantly higher than UPIII expression in conventional BUC (44% vs 17%, P < .001) and UUTUC (67% vs 46%, P = .045). UPIII expression was significantly higher in UUTUC than in BUC (P < .001). In UC variants, UPII expression was significantly higher than UPIII expression in micropapillary (91% vs 25%, P < .001), plasmacytoid (63% vs 6%, P < .001), and sarcomatoid (29% vs 5%, P = .032) variants. Only rare cases of the small cell carcinoma variant had focal UPII and UPIII expression. Compared with conventional UC, the sarcomatoid variant had significantly lower UPII expression, whereas the micropapillary variant had significantly higher UPII expression (P < .001). Conclusions: UPII demonstrates a significantly higher sensitivity than UPIII in conventional and variant UCs. Thus, UPII is a more valuable marker than UPIII in immunohistochemical analyses for confirming the urothelial origin of carcinomas.

Original languageEnglish (US)
Pages (from-to)864-871
Number of pages8
JournalAmerican Journal of Clinical Pathology
Volume142
Issue number6
DOIs
StatePublished - Dec 2014

Keywords

  • Uroplakin II
  • Uroplakin III
  • Urothelial carcinoma
  • Urothelial carcinoma variants

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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