Uptake of silica and carbon nanotubes by human macrophages/ monocytes induces activation of fibroblasts in vitro - Potential implication for pathogenesis of inflammation and fibrotic diseases

X. Guo, Chinnaswamy Jagannath, M. G. Espitia, X. Zhou

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The potential pathogenic effects of silica and carbon nanotubes (CNTs) on fibroblasts, macrophages/ monocytes, and T cells were investigated. Human macrophage/monocytes were cultured and stimulated with silica, CNTs, or titanium particles. After adding human T cells to the stimulated macrophages/ monocytes, the cells were added to cultured human fibroblasts. Upon microscopic examination, CNT stimulation after 24 hours showed centralization of macrophages/monocytes around the CNTs. Silica stimulation showed a significant increase of IL-1a and IL-1β in cultured medium, and an increased gene expression of CTGF in cultured fibroblasts at 1 hour, as well as an up-regulation of the COL1A2 gene at 24-hour time point. In addition to the same changes of IL-Iα, IL-1β and the COL1A2 by silica, CNT stimulation showed an increase of IL-8 in cultured medium at 1-hour time point. Titanium stimulation yielded no significant changes. The results indicate a proinflammatory and/or profibrotic effect of silica and CNTs to cultured human cells including macrophages/monocyte, T cells and fibroblasts.

Original languageEnglish (US)
Pages (from-to)713-719
Number of pages7
JournalInternational Journal of Immunopathology and Pharmacology
Volume25
Issue number3
DOIs
StatePublished - 2012

Keywords

  • CTGF
  • Carbon nano tubes
  • Cytokines
  • Fibrosis
  • Inflammation
  • Silica

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

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