Upregulation of acidic fibroblast growth factor during development of experimental lung fibrosis

Roberto Barrios, Annie Pardo, Carlos Ramos, Martha Montaño, Remedios Ramirez, Moisés Selman

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Fibroblast proliferation and extracellular matrix are crucial in the pathogenesis of lung fibrosis. Fibroblast growth factor (FGF)-1 participates in both processes, but its role in lung fibrogenesis has not been evaluated. We analyzed the expression of FGF-1 and of FGF receptor (FGFR) in a model of lung fibrosis induced in rats with paraquant plus hyperoxia. Experimental and control animals were killed at 48 h and 2, 4, and 8 wk, and the lungs were studied by in situ hybridization, immunohistochemistry, and normal lungs, scattered macrophages contained FGF-1. In contrast, all times examined, the injured lungs exhibited FGF-1 transcript and the immunoreactive protein, mainly in alveolar epithelial cells and macrophages. In advanced fibrotic lesions, fibroblasts also appeared stained. Northern blot corroborated the upregulation of FGF-1 mRNA. FGFR was not observed in normal lungs and was virtually immonolocalized in the same cell types as the corresponding ligand. These findings suggest that FGF-1 and FGFR are actively synthesized during the development of pulmonary fibrosis.

Original languageEnglish (US)
Pages (from-to)L451-L458
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume273
Issue number2 17-2
DOIs
StatePublished - 1997

Keywords

  • Alveolar epithelial cells
  • Collagen
  • Fibroblast growth factors
  • Fibroblast proliferation
  • Pulmonary fibrosis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology
  • Physiology (medical)

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