Unusual form of oxacillin resistance in methicillin-resistant Staphylococcus aureus clinical strains

Betty A. Forbes, Karina Bombicino, Konrad Plata, Arabela Cuirolo, Dawn Webber, Connie L. Bender, Adriana E. Rosato

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

The mechanisms by which there is differential expression of resistance to oxacillin within the populations of a single strain remains to be fully understood. The purpose of this study was to evaluate and characterize 25 GOA48 methicillin-resistant Staphylococcus aureus (MRSA) oxacillin-susceptible mecA-positive strains, which were obtained by screening consecutively 832 S. aureus isolates. These 25 isolates (3% of the total strains investigated) were uniformly detected by extending the 24-h oxacillin agar screen plate to 48 h (namely, GOA48-MRSA). Twenty-two isolates tested positive for penicillin-binding protein 2a, whereas the remaining 3 isolates were inconsistently mecA positive. Inconsistent detection of mecA by polymerase chain reaction (PCR) in the mentioned 3 isolates was investigated by colony hybridization using a mecA probe (≥80% of colonies hybridized poorly to the probe). A PCR product that amplified the empty SCCmec insertion site (attB), present only if the element was excised, resulted positive in all 3 isolates before oxacillin exposure, whereas integrated elements were positive only for oxacillin-grown isolates. The remaining 22 strains did not reveal excision demonstrating stable mecA. We concluded that resistance to β-lactams in MRSA-positive mecA strains susceptible to oxacillin is associated to an extreme heterogeneous expression of resistance combined in some cases to oxacillin SCCmec excision.

Original languageEnglish (US)
Pages (from-to)387-395
Number of pages9
JournalDiagnostic Microbiology and Infectious Disease
Volume61
Issue number4
DOIs
StatePublished - Aug 2008

Keywords

  • Heterogenecity
  • mecA
  • MRSA
  • Oxacillin resistance
  • S. aureus

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Virology
  • Parasitology
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology

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