Unspecific DNA binding of the DNA binding domain of the glucocorticoid receptor studied with flow linear dichroism

Per Hagmar, Karin Dahlman, Masayuki Takahashi, Jan Carlstedt-Duke, Jan Åke Gustafsson, Bengt Nordén

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The unspecific interaction between the DNA-binding domain of the human glucocorticoid receptor and DNA was studied using linear dichroism (LD) and circular dichroism (CD) spectroscopy. The amplitude of the LD signal was found to increase upon addition of protein at ionic strengths less than 60 nM Na+ indicating an increased persistence length of the complex compared to uncomplexed DNA. Analysis of the LD spectrum suggests that the binding does not involve intercalation of tyrosine residues. Evidence of saturation is found at a binding stoichiometry of approximately 5 DNA base pairs per protein monomer.

Original languageEnglish (US)
Pages (from-to)28-32
Number of pages5
JournalFEBS Letters
Volume253
Issue number1-2
DOIs
StatePublished - Aug 14 1989

Keywords

  • DNA-protein interaction
  • Glucocorticoid receptor
  • Linear dichroism

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Fingerprint Dive into the research topics of 'Unspecific DNA binding of the DNA binding domain of the glucocorticoid receptor studied with flow linear dichroism'. Together they form a unique fingerprint.

Cite this