@article{73e8e704f8ca409e9f8e1b35dc8ff930,
title = "Uncapped 5′ ends of mRNAs targeted by cytoplasmic capping map to the vicinity of downstream CAGE tags",
abstract = "In mammalian transcriptomes approximately 25% of 5′ ends determined by Capped Analysis of Gene Expression (CAGE) map to locations within spliced exons. The current study sought to determine if the cytoplasmic capping complex participates in generating these downstream CAGE tags. 5′-RACE was used to amplify the uncapped ends of target transcripts that accumulate when cytoplasmic capping is blocked. Sequencing of these RACE products mapped the positions of uncapped ends either exactly to or just downstream of archived CAGE tags. These findings support a role for cytoplasmic capping in generating the downstream capped ends identified by CAGE.",
keywords = "CAGE, Cytoplasm, Cytoplasmic capping, Mammalian cell, Uncapped 5′ end, mRNA capping",
author = "Kiss, {Daniel L.} and Kenji Oman and Ralf Bundschuh and Schoenberg, {Daniel R.}",
note = "Funding Information: The authors would like to acknowledge Ken Gerien for initial experiments that helped establish ligation conditions for the 5′ RACE reactions and the Fisk Laboratory for providing the tubulin antibody. This work was supported by grants from the National Institutes of Health [Grant Number R01 GM084177 to D.R.S.] and the National Science Foundation [Grant Numbers DMR-1105458 and 1410172 to R.B.]. D.L.K. was supported by a training grant from the National Cancer Institute [Grant Number T32 CA0093338 ] and a Pelotonia postdoctoral fellowship award . The content is solely the responsibility of the authors and does not necessarily represent the official views of Pelotonia, The Ohio State University, the National Science Foundation, or the National Institutes of Health. Publisher Copyright: {\textcopyright} 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2015",
month = jan,
day = "30",
doi = "10.1016/j.febslet.2014.12.009",
language = "English (US)",
volume = "589",
pages = "279--284",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Wiley",
number = "3",
}