TY - JOUR
T1 - Ultrastructural localization of dpnh-diaphorase in the cochlea
AU - Lim, D. J.
N1 - Funding Information:
1 am particularly indebted to Dr. Dante Scarpelli for his critical review of the manuscript and helpful advice. The author wishes to thank Drs. William Saunders and Ryland Mundie for their kind encouragement in conducting this experiment. I wish to express many thanks to Dr. WiIliam MeInick for his stimulating suggestions in writing this paper. The valuable technical assistance was provided by Mrs. Mariann Migliore and Mr. Lawrence Irwin. Miss Barbara Wheaton provided helpful technical advice. Manuscript was typed by Mrs. Phyllis Yamokoski. The research reported in this paper was sponsored in part by the Aerospace Medical Research Laboratories, Aerospace Medical Division, Air Force Systems Command, Wright-Patterson Air Force Base, Ohio, under Contract No. F 33615-68-C-1127, and by the Research Grant from the Bremer Foundation.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1970
Y1 - 1970
N2 - Submicroscopic localization of DPm-diaphorase (NADH-cytochrome C reductase) in the glutaralde-hyde prefixed inner ear of the laboratory animals was studied utilizing TNBT (tetranitro blue tetrazo-lium) as an indicator. The strongest reaction was found in the mitochondria of the outer hair cells. The enzyme reaction was also found on the endoplasmic reticulum of the outer and inner hair cells, epithelial cells of the stria vascularis, Reissner's membrane, and connective tissue cells of the spiral ligament and limbus. Afferent nerve endings demonstrated diffuse enzyme reaction in their cytoplasm, however, efferent nerve endings showed dense reaction along the double membrane located opposite the nerve ending inside of the hair cells. Some of the interdental cells showed reaction in their cytoplasmic contents and showed differences of intensity of reaction among the interdental cells, possibly indicating different stages of enzyme activity.
AB - Submicroscopic localization of DPm-diaphorase (NADH-cytochrome C reductase) in the glutaralde-hyde prefixed inner ear of the laboratory animals was studied utilizing TNBT (tetranitro blue tetrazo-lium) as an indicator. The strongest reaction was found in the mitochondria of the outer hair cells. The enzyme reaction was also found on the endoplasmic reticulum of the outer and inner hair cells, epithelial cells of the stria vascularis, Reissner's membrane, and connective tissue cells of the spiral ligament and limbus. Afferent nerve endings demonstrated diffuse enzyme reaction in their cytoplasm, however, efferent nerve endings showed dense reaction along the double membrane located opposite the nerve ending inside of the hair cells. Some of the interdental cells showed reaction in their cytoplasmic contents and showed differences of intensity of reaction among the interdental cells, possibly indicating different stages of enzyme activity.
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U2 - 10.3109/00016487009123334
DO - 10.3109/00016487009123334
M3 - Article
C2 - 5446608
AN - SCOPUS:0014725962
VL - 69
SP - 32
EP - 45
JO - Acta Oto-Laryngologica
JF - Acta Oto-Laryngologica
SN - 0001-6489
IS - 1-6
ER -