Ultrastructural changes of donor livers in liver transplantation indicate hepatocytes injury

The use of electron microscopy (EM) can provide details about cells and tissue down to the nanometer level. We aim to observe ultrastructural changes in the donor liver by EM and analyze the relationship with prognosis. Data from 89 liver transplant recipients were collected and analyzed for recovery of graft function. The results revealed significantly higher organelle injury scores in the primary liver graft nonfunction (PNF) group. High-score group had higher peak alanine aminotransferases, peak aspartate aminotransferases, and peak international normalized ratio (p =.041,.006 and.036, respectively). Warm ischemia time, score of rough endoplasmic reticulum and nucleus was larger in low-score group (p =.007,.006, and.025, respectively). Patients in high-score group had a significantly short survival time (60.0% vs. 92.9%, p =.0039). No significant difference was found in the analysis of 3-year survival rate (60% vs. 84.5%, p =.07). EM is one of feasible and effective strategy for evaluating the quality of donor liver and the patient's prognosis. Ultrastructural changes under EM indicate hepatocytes injury and a high score indicates a worse outcome in early period but does not affect long-term survival. Research highlights: We showed that ultrastructural changes in the donor liver by electron microscopy indicate hepatocytes injury and could be a reference for prognosis. A high score indicates a worse outcome in early period but does not affect long-term survival. It is rare in the current researches.