Ultrasound mediated gene delivery using bubble containing lipoplexes

Junhua Mai, Liu Rui, Wei Xiaohui, Yao Yanbin, Xu Yuhong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Ultrasound application may be used to drive the entrance of plasmid DNA in vitro and in vivo. For efficient transfection, air bubble containing contrast agents are usually used. We described here a new method to produce air bubble containing lipid/DNA lipoplexes. The transfection efficiencies using these lipoplexes were examined in vitro on SPCA-1 cells. The cells were irradiated with 1 MHz ultrasound for various time durations. Parameters such as ultrasound intensity, sonication time, and ultrasound frequency used were evaluated for improved transfection.

Original languageEnglish (US)
Title of host publicationICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering
Pages485-489
Number of pages5
DOIs
StatePublished - Dec 1 2006
EventICBPE 2006 - 2006 International Conference on Biomedical and Pharmaceutical Engineering - Singapore, Singapore
Duration: Dec 11 2006Dec 14 2006

Other

OtherICBPE 2006 - 2006 International Conference on Biomedical and Pharmaceutical Engineering
CountrySingapore
CitySingapore
Period12/11/0612/14/06

Keywords

  • Gene delivery
  • Liposome
  • Sonoporation
  • Ultrasound contrast agent

ASJC Scopus subject areas

  • Biomedical Engineering
  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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