Abstract
Ultrasound application may be used to drive the entrance of plasmid DNA in vitro and in vivo. For efficient transfection, air bubble containing contrast agents are usually used. We described here a new method to produce air bubble containing lipid/DNA lipoplexes. The transfection efficiencies using these lipoplexes were examined in vitro on SPCA-1 cells. The cells were irradiated with 1 MHz ultrasound for various time durations. Parameters such as ultrasound intensity, sonication time, and ultrasound frequency used were evaluated for improved transfection.
Original language | English (US) |
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Title of host publication | ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering |
Pages | 485-489 |
Number of pages | 5 |
DOIs | |
State | Published - Dec 1 2006 |
Event | ICBPE 2006 - 2006 International Conference on Biomedical and Pharmaceutical Engineering - Singapore, Singapore Duration: Dec 11 2006 → Dec 14 2006 |
Other
Other | ICBPE 2006 - 2006 International Conference on Biomedical and Pharmaceutical Engineering |
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Country | Singapore |
City | Singapore |
Period | 12/11/06 → 12/14/06 |
Keywords
- Gene delivery
- Liposome
- Sonoporation
- Ultrasound contrast agent
ASJC Scopus subject areas
- Biomedical Engineering
- Pharmacology (medical)
- Pharmacology, Toxicology and Pharmaceutics(all)