Ultrasound mediated gene delivery using bubble containing lipoplexes

Junhua Mai; Liu Rui; Wei Xiaohui; Yao Yanbin; Xu Yuhong

doi:10.1109/ICBPE.2006.348642

Ultrasound mediated gene delivery using bubble containing lipoplexes

Junhua Mai, Liu Rui, Wei Xiaohui, Yao Yanbin, Xu Yuhong

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Ultrasound application may be used to drive the entrance of plasmid DNA in vitro and in vivo. For efficient transfection, air bubble containing contrast agents are usually used. We described here a new method to produce air bubble containing lipid/DNA lipoplexes. The transfection efficiencies using these lipoplexes were examined in vitro on SPCA-1 cells. The cells were irradiated with 1 MHz ultrasound for various time durations. Parameters such as ultrasound intensity, sonication time, and ultrasound frequency used were evaluated for improved transfection.

Original language	English (US)
Title of host publication	ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering
Pages	485-489
Number of pages	5
DOIs	https://doi.org/10.1109/ICBPE.2006.348642
State	Published - Dec 1 2006
Event	ICBPE 2006 - 2006 International Conference on Biomedical and Pharmaceutical Engineering - Singapore, Singapore Duration: Dec 11 2006 → Dec 14 2006

Other

Other	ICBPE 2006 - 2006 International Conference on Biomedical and Pharmaceutical Engineering
Country	Singapore
City	Singapore
Period	12/11/06 → 12/14/06

Keywords

Gene delivery
Liposome
Sonoporation
Ultrasound contrast agent

ASJC Scopus subject areas

Biomedical Engineering
Pharmacology (medical)
Pharmacology, Toxicology and Pharmaceutics(all)

Access to Document

10.1109/ICBPE.2006.348642

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Cite this

Mai, J, Rui, L, Xiaohui, W, Yanbin, Y & Yuhong, X 2006, Ultrasound mediated gene delivery using bubble containing lipoplexes. in ICBPE 2006 - Proceedings of the 2006 International Conference on Biomedical and Pharmaceutical Engineering., 4155951, pp. 485-489, ICBPE 2006 - 2006 International Conference on Biomedical and Pharmaceutical Engineering, Singapore, Singapore, 12/11/06. https://doi.org/10.1109/ICBPE.2006.348642

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abstract = "Ultrasound application may be used to drive the entrance of plasmid DNA in vitro and in vivo. For efficient transfection, air bubble containing contrast agents are usually used. We described here a new method to produce air bubble containing lipid/DNA lipoplexes. The transfection efficiencies using these lipoplexes were examined in vitro on SPCA-1 cells. The cells were irradiated with 1 MHz ultrasound for various time durations. Parameters such as ultrasound intensity, sonication time, and ultrasound frequency used were evaluated for improved transfection.",

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author = "Junhua Mai and Liu Rui and Wei Xiaohui and Yao Yanbin and Xu Yuhong",

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