TY - JOUR
T1 - Ultrasonic processing of enzymes
T2 - Effect on enzymatic activity of glucose oxidase
AU - Guiseppi-Elie, Anthony
AU - Choi, Sung Ho
AU - Geckeler, Kurt E.
N1 - Funding Information:
This work was supported by the US Department of Defense (DoDPRMRP) grant PR023081/DAMD17-03-1-0172 and by the Consortium of the Clemson University Center for Bioelectronics, Biosensors and Biochips. KEG thanks the Clemson C3B for a Visiting Professorship and SHC for a transient graduate studentship. In addition, SHC acknowledges financial support from the Dasan Global Explorer Program (GIST, South Korea).
PY - 2009/6
Y1 - 2009/6
N2 - The effect of ultrasonication on the enzymatic stability, conformation, and catalytic activity of the important oxidoreductase, glucose oxidase (GOx), was investigated. Thus, buffer-free aqueous solutions of GOx were ultrasonicated (23 kHz at 4 °C) for different periods of time (10, 30, and 60 min) and studied in terms of their enzymatic activity. The ultrasonicated GOx was also studied by UV/vis and circular dichroism (CD) spectroscopy and by thermogravimetric analysis, and compared with pristine GOx. The CD spectra of ultrasonicated GOx showed a different composition with reduced α-helix and β-sheet fractions upon extended sonication compared with the pristine GOx. Along with the changes of the secondary structure, the enzymatic activity measured via HRP-coupled bioassay of the sonicated GOx showed a small corresponding decrease. Low temperature ultrasonic processing of GOx does not appreciably compromise bioactivity.
AB - The effect of ultrasonication on the enzymatic stability, conformation, and catalytic activity of the important oxidoreductase, glucose oxidase (GOx), was investigated. Thus, buffer-free aqueous solutions of GOx were ultrasonicated (23 kHz at 4 °C) for different periods of time (10, 30, and 60 min) and studied in terms of their enzymatic activity. The ultrasonicated GOx was also studied by UV/vis and circular dichroism (CD) spectroscopy and by thermogravimetric analysis, and compared with pristine GOx. The CD spectra of ultrasonicated GOx showed a different composition with reduced α-helix and β-sheet fractions upon extended sonication compared with the pristine GOx. Along with the changes of the secondary structure, the enzymatic activity measured via HRP-coupled bioassay of the sonicated GOx showed a small corresponding decrease. Low temperature ultrasonic processing of GOx does not appreciably compromise bioactivity.
KW - Enzyme activity
KW - Enzyme processing
KW - Glucose oxidase
KW - Ultrasonication
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U2 - 10.1016/j.molcatb.2008.12.005
DO - 10.1016/j.molcatb.2008.12.005
M3 - Article
AN - SCOPUS:63249116554
SN - 1381-1177
VL - 58
SP - 118
EP - 123
JO - Journal of Molecular Catalysis B: Enzymatic
JF - Journal of Molecular Catalysis B: Enzymatic
IS - 1-4
ER -