Ultrasensitive RNA in situ hybridization for detection of restricted clonal expression of low-abundance immunoglobulin light chain mRNA in B-cell lymphoproliferative disorders

Raymond R. Tubbs; Hongwei Wang; Zhen Wang; Eugen C. Minca; Bryce P. Portier; Aaron M. Gruver; Christopher Lanigan; Yuling Luo; James R. Cook; Xiao Jun Ma

doi:10.1309/AJCPJTWK07FSABRJ

Ultrasensitive RNA in situ hybridization for detection of restricted clonal expression of low-abundance immunoglobulin light chain mRNA in B-cell lymphoproliferative disorders

Raymond R. Tubbs, Hongwei Wang, Zhen Wang, Eugen C. Minca, Bryce P. Portier, Aaron M. Gruver, Christopher Lanigan, Yuling Luo, James R. Cook, Xiao Jun Ma

Academic Institute

Research output: Contribution to journal › Article

23 Scopus citations

Abstract

Objectives: To assess the feasibility of using a novel ultrasensitive bright-field in situ hybridization approach (BRISH) to evaluate k and l immunoglobulin messenger RNA (mRNA) expression in situ in B-cell non-Hodgkin lymphoma (NHL). Methods: A series of 110 semiconsecutive clinical cases evaluated for lymphoma with historic flow cytometric (FCM) results were assessed with BRISH. Results: BRISH light chain restriction (LCR) results were concordant with FCM in 108 (99%) of 109 evaluable cases. Additional small B-cell lymphoma cohorts were successfully evaluated. Conclusions: BRISH analysis of k and l immunoglobulin mRNA expression is a sensitive tool for establishing LCR in B-cell NHL when FCM results are not available.

Original language	English (US)
Pages (from-to)	736-746
Number of pages	11
Journal	American Journal of Clinical Pathology
Volume	140
Issue number	5
DOIs	https://doi.org/10.1309/AJCPJTWK07FSABRJ
State	Published - Nov 2013

Keywords

B-cell clonality
B-cell lymphoma
BRISH
CISH
In situ hybridization
RNAscope

ASJC Scopus subject areas

Pathology and Forensic Medicine

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Tubbs, R. R., Wang, H., Wang, Z., Minca, E. C., Portier, B. P., Gruver, A. M., Lanigan, C., Luo, Y., Cook, J. R., & Ma, X. J. (2013). Ultrasensitive RNA in situ hybridization for detection of restricted clonal expression of low-abundance immunoglobulin light chain mRNA in B-cell lymphoproliferative disorders. American Journal of Clinical Pathology, 140(5), 736-746. https://doi.org/10.1309/AJCPJTWK07FSABRJ

Ultrasensitive RNA in situ hybridization for detection of restricted clonal expression of low-abundance immunoglobulin light chain mRNA in B-cell lymphoproliferative disorders. / Tubbs, Raymond R.; Wang, Hongwei; Wang, Zhen; Minca, Eugen C.; Portier, Bryce P.; Gruver, Aaron M.; Lanigan, Christopher; Luo, Yuling; Cook, James R.; Ma, Xiao Jun.

In: American Journal of Clinical Pathology, Vol. 140, No. 5, 11.2013, p. 736-746.

Research output: Contribution to journal › Article

Tubbs, RR, Wang, H, Wang, Z, Minca, EC, Portier, BP, Gruver, AM, Lanigan, C, Luo, Y, Cook, JR & Ma, XJ 2013, 'Ultrasensitive RNA in situ hybridization for detection of restricted clonal expression of low-abundance immunoglobulin light chain mRNA in B-cell lymphoproliferative disorders', American Journal of Clinical Pathology, vol. 140, no. 5, pp. 736-746. https://doi.org/10.1309/AJCPJTWK07FSABRJ

Tubbs, Raymond R. ; Wang, Hongwei ; Wang, Zhen ; Minca, Eugen C. ; Portier, Bryce P. ; Gruver, Aaron M. ; Lanigan, Christopher ; Luo, Yuling ; Cook, James R. ; Ma, Xiao Jun. / Ultrasensitive RNA in situ hybridization for detection of restricted clonal expression of low-abundance immunoglobulin light chain mRNA in B-cell lymphoproliferative disorders. In: American Journal of Clinical Pathology. 2013 ; Vol. 140, No. 5. pp. 736-746.

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abstract = "Objectives: To assess the feasibility of using a novel ultrasensitive bright-field in situ hybridization approach (BRISH) to evaluate k and l immunoglobulin messenger RNA (mRNA) expression in situ in B-cell non-Hodgkin lymphoma (NHL). Methods: A series of 110 semiconsecutive clinical cases evaluated for lymphoma with historic flow cytometric (FCM) results were assessed with BRISH. Results: BRISH light chain restriction (LCR) results were concordant with FCM in 108 (99%) of 109 evaluable cases. Additional small B-cell lymphoma cohorts were successfully evaluated. Conclusions: BRISH analysis of k and l immunoglobulin mRNA expression is a sensitive tool for establishing LCR in B-cell NHL when FCM results are not available.",

keywords = "B-cell clonality, B-cell lymphoma, BRISH, CISH, In situ hybridization, RNAscope",

author = "Tubbs, {Raymond R.} and Hongwei Wang and Zhen Wang and Minca, {Eugen C.} and Portier, {Bryce P.} and Gruver, {Aaron M.} and Christopher Lanigan and Yuling Luo and Cook, {James R.} and Ma, {Xiao Jun}",

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AU - Minca, Eugen C.

AU - Portier, Bryce P.

AU - Gruver, Aaron M.

AU - Lanigan, Christopher

AU - Luo, Yuling

AU - Cook, James R.

AU - Ma, Xiao Jun

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KW - In situ hybridization

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