Ultrasensitive, multiplex Raman frequency shift immunoassay of liver cancer biomarkers in physiological media

Bochong Tang, Jiaojiao Wang, James A. Hutchison, Lei Ma, Ning Zhang, Hua Guo, Zhongbo Hu, Min Li, Yuliang Zhao

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

Highly sensitive multiplex biomarker detection is critical for the early diagnosis of liver cancer. Here, a surface-enhanced Raman scattering (SERS) frequency-shift immunoassay is developed for detection of liver cancer biomarkers α-fetoprotein and Glypican-3 down to subpicomolar concentrations in saline solution. A high temperature modification of the Tollen's method affords silver nanoparticle films with excellent SERS response upon which ordered domains of Raman reporters are chemisorbed by microcontact printing. Shifts in the reporters SERS spectrum in response to a bound antibody's biomarker recognition constitutes the frequency shift assay, exhibiting here exceptional sensitivity and specificity and shown to function in fetal calf serum and in the serum of a patient with hepatocellular carcinoma.

Original languageEnglish (US)
Pages (from-to)871-879
Number of pages9
JournalACS Nano
Volume10
Issue number1
DOIs
StatePublished - Jan 26 2016

Keywords

  • Frequency shift immunoassay
  • Liver cancer biomarkers
  • Microcontact printing (μ CP)
  • Multiplex detection
  • SERS

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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