Ultrasensitive, multiplex Raman frequency shift immunoassay of liver cancer biomarkers in physiological media

Bochong Tang; Jiaojiao Wang; James A. Hutchison; Lei Ma; Ning Zhang; Hua Guo; Zhongbo Hu; Min Li; Yuliang Zhao

doi:10.1021/acsnano.5b06007

Ultrasensitive, multiplex Raman frequency shift immunoassay of liver cancer biomarkers in physiological media

Bochong Tang, Jiaojiao Wang, James A. Hutchison, Lei Ma, Ning Zhang, Hua Guo, Zhongbo Hu, Min Li, Yuliang Zhao

Houston Methodist

Research output: Contribution to journal › Article › peer-review

96 Scopus citations

Abstract

Highly sensitive multiplex biomarker detection is critical for the early diagnosis of liver cancer. Here, a surface-enhanced Raman scattering (SERS) frequency-shift immunoassay is developed for detection of liver cancer biomarkers α-fetoprotein and Glypican-3 down to subpicomolar concentrations in saline solution. A high temperature modification of the Tollen's method affords silver nanoparticle films with excellent SERS response upon which ordered domains of Raman reporters are chemisorbed by microcontact printing. Shifts in the reporters SERS spectrum in response to a bound antibody's biomarker recognition constitutes the frequency shift assay, exhibiting here exceptional sensitivity and specificity and shown to function in fetal calf serum and in the serum of a patient with hepatocellular carcinoma.

Original language	English (US)
Pages (from-to)	871-879
Number of pages	9
Journal	ACS Nano
Volume	10
Issue number	1
DOIs	https://doi.org/10.1021/acsnano.5b06007
State	Published - Jan 26 2016

Keywords

Frequency shift immunoassay
Liver cancer biomarkers
Microcontact printing (μ CP)
Multiplex detection
SERS

ASJC Scopus subject areas

Materials Science(all)
Engineering(all)
Physics and Astronomy(all)

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10.1021/acsnano.5b06007

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title = "Ultrasensitive, multiplex Raman frequency shift immunoassay of liver cancer biomarkers in physiological media",

abstract = "Highly sensitive multiplex biomarker detection is critical for the early diagnosis of liver cancer. Here, a surface-enhanced Raman scattering (SERS) frequency-shift immunoassay is developed for detection of liver cancer biomarkers α-fetoprotein and Glypican-3 down to subpicomolar concentrations in saline solution. A high temperature modification of the Tollen's method affords silver nanoparticle films with excellent SERS response upon which ordered domains of Raman reporters are chemisorbed by microcontact printing. Shifts in the reporters SERS spectrum in response to a bound antibody's biomarker recognition constitutes the frequency shift assay, exhibiting here exceptional sensitivity and specificity and shown to function in fetal calf serum and in the serum of a patient with hepatocellular carcinoma.",

keywords = "Frequency shift immunoassay, Liver cancer biomarkers, Microcontact printing (μ CP), Multiplex detection, SERS",

author = "Bochong Tang and Jiaojiao Wang and Hutchison, {James A.} and Lei Ma and Ning Zhang and Hua Guo and Zhongbo Hu and Min Li and Yuliang Zhao",

note = "Funding Information: This work was supported in part by the National Basic Research Program of China (no. 2011CB933101, 2015CB932004), National Natural Science Foundation of China (grant no. 21303208). Publisher Copyright: {\textcopyright} 2015 American Chemical Society. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.",

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doi = "10.1021/acsnano.5b06007",

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AU - Tang, Bochong

AU - Wang, Jiaojiao

AU - Hutchison, James A.

AU - Ma, Lei

AU - Zhang, Ning

AU - Guo, Hua

AU - Hu, Zhongbo

AU - Li, Min

AU - Zhao, Yuliang

N1 - Funding Information: This work was supported in part by the National Basic Research Program of China (no. 2011CB933101, 2015CB932004), National Natural Science Foundation of China (grant no. 21303208). Publisher Copyright: © 2015 American Chemical Society. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.

PY - 2016/1/26

Y1 - 2016/1/26

N2 - Highly sensitive multiplex biomarker detection is critical for the early diagnosis of liver cancer. Here, a surface-enhanced Raman scattering (SERS) frequency-shift immunoassay is developed for detection of liver cancer biomarkers α-fetoprotein and Glypican-3 down to subpicomolar concentrations in saline solution. A high temperature modification of the Tollen's method affords silver nanoparticle films with excellent SERS response upon which ordered domains of Raman reporters are chemisorbed by microcontact printing. Shifts in the reporters SERS spectrum in response to a bound antibody's biomarker recognition constitutes the frequency shift assay, exhibiting here exceptional sensitivity and specificity and shown to function in fetal calf serum and in the serum of a patient with hepatocellular carcinoma.

AB - Highly sensitive multiplex biomarker detection is critical for the early diagnosis of liver cancer. Here, a surface-enhanced Raman scattering (SERS) frequency-shift immunoassay is developed for detection of liver cancer biomarkers α-fetoprotein and Glypican-3 down to subpicomolar concentrations in saline solution. A high temperature modification of the Tollen's method affords silver nanoparticle films with excellent SERS response upon which ordered domains of Raman reporters are chemisorbed by microcontact printing. Shifts in the reporters SERS spectrum in response to a bound antibody's biomarker recognition constitutes the frequency shift assay, exhibiting here exceptional sensitivity and specificity and shown to function in fetal calf serum and in the serum of a patient with hepatocellular carcinoma.

KW - Frequency shift immunoassay

KW - Liver cancer biomarkers

KW - Microcontact printing (μ CP)

KW - Multiplex detection

KW - SERS

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Ultrasensitive, multiplex Raman frequency shift immunoassay of liver cancer biomarkers in physiological media

Abstract

Keywords

ASJC Scopus subject areas

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