TY - JOUR
T1 - Ultrasensitive Detection of COVID-19 Virus N Protein Based on p-Toluenesulfonyl Modified Fluorescent Microspheres Immunoassay
AU - Mao, Mao
AU - Wu, Feng
AU - Shi, Xueying
AU - Huang, Yulan
AU - Ma, Lan
N1 - Funding Information:
This work was supported by the National Key R&D Program of China (2020YFA0908900). Shen zhen Science and Technology research and development funds (JCYJ20200109143018683). State Key Laboratory of Chemical Oncogenomics, Institute of Biomedical Health Technology and Engineer-ing, Shenzhen Bay Laboratory.
Funding Information:
Funding: This work was supported by the National Key R&D Program of China (2020YFA0908900). Shen zhen Science and Technology research and development funds (JCYJ20200109143018683). State Key Laboratory of Chemical Oncogenomics, Institute of Biomedical Health Technology and Engineering, Shenzhen Bay Laboratory.
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/7
Y1 - 2022/7
N2 - The pandemic of new coronary pneumonia caused by the COVID-19 virus continues to ravage the world. Large-scale population testing is the key to controlling infection and related mortality worldwide. Lateral flow immunochromatographic assay (LFIA) is fast, inexpensive, simple to operate, and easy to carry, very suitable for detection sites. This study developed a COVID-19 N protein detect strip based on p-toluenesulfonyl modified rare earth fluorescent microspheres. The p-toluenesulfonyl-activated nanomaterials provide reactive sulfonyl esters to covalently attach antibodies or other ligands containing primary amino or sulfhydryl groups to the nanomaterial surface. Antibodies are immobilized on these nanomaterials through the Fc region, which ensures optimal orientation of the antibody, thereby increasing the capture rate of the target analyte. The use of buffers with high ionic strength can promote hydrophobic binding; in addition, higher pH could promote the reactivity of the tosyl group. The detection limit of the prepared COVID-19 N protein strips can reach 0.01 ng/mL, so it has great application potential in large-scale population screening.
AB - The pandemic of new coronary pneumonia caused by the COVID-19 virus continues to ravage the world. Large-scale population testing is the key to controlling infection and related mortality worldwide. Lateral flow immunochromatographic assay (LFIA) is fast, inexpensive, simple to operate, and easy to carry, very suitable for detection sites. This study developed a COVID-19 N protein detect strip based on p-toluenesulfonyl modified rare earth fluorescent microspheres. The p-toluenesulfonyl-activated nanomaterials provide reactive sulfonyl esters to covalently attach antibodies or other ligands containing primary amino or sulfhydryl groups to the nanomaterial surface. Antibodies are immobilized on these nanomaterials through the Fc region, which ensures optimal orientation of the antibody, thereby increasing the capture rate of the target analyte. The use of buffers with high ionic strength can promote hydrophobic binding; in addition, higher pH could promote the reactivity of the tosyl group. The detection limit of the prepared COVID-19 N protein strips can reach 0.01 ng/mL, so it has great application potential in large-scale population screening.
KW - COVID-19 N protein
KW - fluorescent microspheres
KW - lateral flow immunochromatographic assay
KW - p-toluenesulfonyl
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U2 - 10.3390/bios12070437
DO - 10.3390/bios12070437
M3 - Article
C2 - 35884241
AN - SCOPUS:85133181306
VL - 12
JO - Biosensors
JF - Biosensors
SN - 2079-6374
IS - 7
M1 - 437
ER -