Ultra-high resolution imaging of DNA and nucleosomes using non-contact atomic force microscopy

Emma Davies; Kar Seng Teng; Robert Steven Conlan; Stephen Patrick Wilks

doi:10.1016/j.febslet.2005.02.028

Ultra-high resolution imaging of DNA and nucleosomes using non-contact atomic force microscopy

Emma Davies, Kar Seng Teng, Robert Steven Conlan, Stephen Patrick Wilks

Houston Methodist

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Visualisation of nano-scale biomolecules aids understanding and development in molecular biology and nanotechnology. Detailed structure of nucleosomes adsorbed to mica has been captured in the absence of chemical-anchoring techniques, demonstrating the usefulness of non-contact atomic force microscopy (NC-AFM) for ultra-high resolution biomolecular imaging. NC-AFM offers significant advantages in terms of resolution, speed and ease of sample preparation when compared to techniques such as cryo-electron microscopy and X-ray crystallography. In the absence of chemical modification, detailed structure of DNA deposited on a gold substrate was observed for the first time using NC-AFM, opening up possibilities for investigating the electrical properties of unmodified DNA.

Original language	English (US)
Pages (from-to)	1702-1706
Number of pages	5
Journal	FEBS Letters
Volume	579
Issue number	7
DOIs	https://doi.org/10.1016/j.febslet.2005.02.028
State	Published - Mar 14 2005

Keywords

Atomic force
MeSH: DNA
Microscopy
Molecular biology
Molecular structure
Nanotechnology
Nucleosome

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/j.febslet.2005.02.028

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title = "Ultra-high resolution imaging of DNA and nucleosomes using non-contact atomic force microscopy",

abstract = "Visualisation of nano-scale biomolecules aids understanding and development in molecular biology and nanotechnology. Detailed structure of nucleosomes adsorbed to mica has been captured in the absence of chemical-anchoring techniques, demonstrating the usefulness of non-contact atomic force microscopy (NC-AFM) for ultra-high resolution biomolecular imaging. NC-AFM offers significant advantages in terms of resolution, speed and ease of sample preparation when compared to techniques such as cryo-electron microscopy and X-ray crystallography. In the absence of chemical modification, detailed structure of DNA deposited on a gold substrate was observed for the first time using NC-AFM, opening up possibilities for investigating the electrical properties of unmodified DNA.",

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T1 - Ultra-high resolution imaging of DNA and nucleosomes using non-contact atomic force microscopy

AU - Davies, Emma

AU - Teng, Kar Seng

AU - Conlan, Robert Steven

AU - Wilks, Stephen Patrick

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AB - Visualisation of nano-scale biomolecules aids understanding and development in molecular biology and nanotechnology. Detailed structure of nucleosomes adsorbed to mica has been captured in the absence of chemical-anchoring techniques, demonstrating the usefulness of non-contact atomic force microscopy (NC-AFM) for ultra-high resolution biomolecular imaging. NC-AFM offers significant advantages in terms of resolution, speed and ease of sample preparation when compared to techniques such as cryo-electron microscopy and X-ray crystallography. In the absence of chemical modification, detailed structure of DNA deposited on a gold substrate was observed for the first time using NC-AFM, opening up possibilities for investigating the electrical properties of unmodified DNA.

KW - Atomic force

KW - MeSH: DNA

KW - Microscopy

KW - Molecular biology

KW - Molecular structure

KW - Nanotechnology

KW - Nucleosome

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Ultra-high resolution imaging of DNA and nucleosomes using non-contact atomic force microscopy

Abstract

Keywords

ASJC Scopus subject areas

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