Two distinct cytokinesis pathways drive trypanosome cell division initiation from opposite cell ends

Qing Zhou, Jianhua Gu, Zhao Rong Lun, Francisco J. Ayala, Ziyin Li

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Cytokinesis in Trypanosoma brucei, an early branching protozoan, occurs along its longitudinal axis uni-directionally from the anterior tip of the new flagellum attachment zone filament toward the cell's posterior end. However, the underlying mechanisms remain elusive. Here we report that cytokinesis in T. brucei is regulated by a concerted action of Polo-like kinase, Aurora B kinase, and a trypanosome- specific protein CIF1. Phosphorylation of CIF1 by Polo-like kinase targets it to the anterior tip of the new flagellum attachment zone filament, where it subsequently recruits Aurora B kinase to initiate cytokinesis. Consistent with its role, CIF1 depletion inhibits cytokinesis initiation from the anterior end of the cell, but, surprisingly, triggers cytokinesis initiation from the posterior end of the cell, suggesting the activation of an alternative cytokinesis from the opposite cell end. Our results reveal the mechanistic roles of CIF1 and Polo-like kinase in cytokinesis initiation and elucidate the mechanism underlying the recruitment of Aurora B kinase to the cytokinesis initiation site at late anaphase. These findings also delineate a signaling cascade controlling cytokinesis initiation from the anterior end of the cell and uncover a backup cytokinesis that is initiated from the posterior end of the cell when the typical anterior-to-posterior cytokinesis is compromised.

Original languageEnglish (US)
Pages (from-to)3287-3292
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number12
DOIs
StatePublished - Mar 22 2016

Keywords

  • Aurora B kinase
  • Backup cytokinesis
  • Cytokinesis
  • Polo-like kinase
  • Trypanosoma brucei

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'Two distinct cytokinesis pathways drive trypanosome cell division initiation from opposite cell ends'. Together they form a unique fingerprint.

Cite this