Tualang honey supplement improves memory performance and hippocampal morphology in stressed ovariectomized rats

Badriya Al-Rahbi; Rahimah Zakaria; Zahiruddin Othman; Asma Hassan; Zul Izhar Mohd Ismail; Sangu Muthuraju

doi:10.1016/j.acthis.2013.05.004

Tualang honey supplement improves memory performance and hippocampal morphology in stressed ovariectomized rats

Badriya Al-Rahbi, Rahimah Zakaria, Zahiruddin Othman, Asma Hassan, Zul Izhar Mohd Ismail, Sangu Muthuraju

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Recently, our research team has reported that Tualang honey was able to improve immediate memory in postmenopausal women comparable with that of estrogen progestin therapy. Therefore the aim of the present study was to examine the effects of Tualang honey supplement on hippocampal morphology and memory performance in ovariectomized (OVX) rats exposed to social instability stress. Female Sprague-Dawley rats were divided into six groups: (i) sham-operated controls, (ii) stressed sham-operated controls, (iii) OVX rats, (iv) stressed OVX rats, (v) stressed OVX rats treated with 17β-estradiol (E2), and (vi) stressed OVX rats treated with Tualang honey. These rats were subjected to social instability stress procedure followed by novel object recognition (NOR) test. Right brain hemispheres were subjected to Nissl staining. The number and arrangement of pyramidal neurons in regions of CA1, CA2, CA3 and the dentate gyrus (DG) were recorded. Two-way ANOVA analyses showed significant interactions between stress and OVX in both STM and LTM test as well as number of Nissl-positive cells in all hippocampal regions. Both E2 and Tualang honey treatments improved both short-term and long-term memory and enhanced the neuronal proliferation of hippocampal CA2, CA3 and DG regions compared to that of untreated stressed OVX rats.

Original language	English (US)
Pages (from-to)	79-88
Number of pages	10
Journal	Acta Histochemica
Volume	116
Issue number	1
DOIs	https://doi.org/10.1016/j.acthis.2013.05.004
State	Published - Jan 2014

Keywords

Estrogen
Hippocampus
Novel object recognition test
Ovariectomy
Rat
Social instability stress
Tualang honey

ASJC Scopus subject areas

Histology
Cell Biology

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10.1016/j.acthis.2013.05.004

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title = "Tualang honey supplement improves memory performance and hippocampal morphology in stressed ovariectomized rats",

abstract = "Recently, our research team has reported that Tualang honey was able to improve immediate memory in postmenopausal women comparable with that of estrogen progestin therapy. Therefore the aim of the present study was to examine the effects of Tualang honey supplement on hippocampal morphology and memory performance in ovariectomized (OVX) rats exposed to social instability stress. Female Sprague-Dawley rats were divided into six groups: (i) sham-operated controls, (ii) stressed sham-operated controls, (iii) OVX rats, (iv) stressed OVX rats, (v) stressed OVX rats treated with 17β-estradiol (E2), and (vi) stressed OVX rats treated with Tualang honey. These rats were subjected to social instability stress procedure followed by novel object recognition (NOR) test. Right brain hemispheres were subjected to Nissl staining. The number and arrangement of pyramidal neurons in regions of CA1, CA2, CA3 and the dentate gyrus (DG) were recorded. Two-way ANOVA analyses showed significant interactions between stress and OVX in both STM and LTM test as well as number of Nissl-positive cells in all hippocampal regions. Both E2 and Tualang honey treatments improved both short-term and long-term memory and enhanced the neuronal proliferation of hippocampal CA2, CA3 and DG regions compared to that of untreated stressed OVX rats.",

keywords = "Estrogen, Hippocampus, Novel object recognition test, Ovariectomy, Rat, Social instability stress, Tualang honey",

author = "Badriya Al-Rahbi and Rahimah Zakaria and Zahiruddin Othman and Asma Hassan and {Mohd Ismail}, {Zul Izhar} and Sangu Muthuraju",

note = "Funding Information: This research was supported by the short-term grant Universiti Sains Malaysia ( 304/PPSP/61311070 ) and Ministry of Health of Oman . We would like to thank Ms. Wan Arfah Nadiah and Ms. Anis Kausar for their assistance with statistical analyses.",

year = "2014",

month = jan,

doi = "10.1016/j.acthis.2013.05.004",

language = "English (US)",

volume = "116",

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AU - Al-Rahbi, Badriya

AU - Zakaria, Rahimah

AU - Othman, Zahiruddin

AU - Hassan, Asma

AU - Mohd Ismail, Zul Izhar

AU - Muthuraju, Sangu

N1 - Funding Information: This research was supported by the short-term grant Universiti Sains Malaysia ( 304/PPSP/61311070 ) and Ministry of Health of Oman . We would like to thank Ms. Wan Arfah Nadiah and Ms. Anis Kausar for their assistance with statistical analyses.

PY - 2014/1

Y1 - 2014/1

N2 - Recently, our research team has reported that Tualang honey was able to improve immediate memory in postmenopausal women comparable with that of estrogen progestin therapy. Therefore the aim of the present study was to examine the effects of Tualang honey supplement on hippocampal morphology and memory performance in ovariectomized (OVX) rats exposed to social instability stress. Female Sprague-Dawley rats were divided into six groups: (i) sham-operated controls, (ii) stressed sham-operated controls, (iii) OVX rats, (iv) stressed OVX rats, (v) stressed OVX rats treated with 17β-estradiol (E2), and (vi) stressed OVX rats treated with Tualang honey. These rats were subjected to social instability stress procedure followed by novel object recognition (NOR) test. Right brain hemispheres were subjected to Nissl staining. The number and arrangement of pyramidal neurons in regions of CA1, CA2, CA3 and the dentate gyrus (DG) were recorded. Two-way ANOVA analyses showed significant interactions between stress and OVX in both STM and LTM test as well as number of Nissl-positive cells in all hippocampal regions. Both E2 and Tualang honey treatments improved both short-term and long-term memory and enhanced the neuronal proliferation of hippocampal CA2, CA3 and DG regions compared to that of untreated stressed OVX rats.

AB - Recently, our research team has reported that Tualang honey was able to improve immediate memory in postmenopausal women comparable with that of estrogen progestin therapy. Therefore the aim of the present study was to examine the effects of Tualang honey supplement on hippocampal morphology and memory performance in ovariectomized (OVX) rats exposed to social instability stress. Female Sprague-Dawley rats were divided into six groups: (i) sham-operated controls, (ii) stressed sham-operated controls, (iii) OVX rats, (iv) stressed OVX rats, (v) stressed OVX rats treated with 17β-estradiol (E2), and (vi) stressed OVX rats treated with Tualang honey. These rats were subjected to social instability stress procedure followed by novel object recognition (NOR) test. Right brain hemispheres were subjected to Nissl staining. The number and arrangement of pyramidal neurons in regions of CA1, CA2, CA3 and the dentate gyrus (DG) were recorded. Two-way ANOVA analyses showed significant interactions between stress and OVX in both STM and LTM test as well as number of Nissl-positive cells in all hippocampal regions. Both E2 and Tualang honey treatments improved both short-term and long-term memory and enhanced the neuronal proliferation of hippocampal CA2, CA3 and DG regions compared to that of untreated stressed OVX rats.

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ER -

Tualang honey supplement improves memory performance and hippocampal morphology in stressed ovariectomized rats

Abstract

Keywords

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