TRIM9 short isoform preferentially promotes DNA and RNA virus-induced production of type I interferon by recruiting GSK3β to TBK1

Yunfei Qin, Qingxiang Liu, Shuo Tian, Weihong Xie, Jun Cui, Rong Fu Wang

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Type I interferon (IFN) is an important component of antiviral innate immune signaling mediated by viral DNA and RNA recognition by the DNA sensor cGAS and RNA sensors RIG-I and MDA5. Activation of these DNA and RNA sensors leads to the recruitment of STING and MAVS, respectively, and converges on TANK-binding kinase 1 (TBK1) signaling for subsequent phosphorylation of IFN regulatory factor 3 (IRF3). However, the mechanisms that control TBK1 activation are still poorly defined. Here, we identify tripartite motif 9 short isoform (TRIM9s) as a positive regulator in type I IFN signaling. Upon viral infection, TRIM9s undergoes Lys-63-linked auto-polyubiquitination and serves as a platform to bridge GSK3β to TBK1, leading to the activation of IRF3 signaling. Interestingly, we found that TRIM9s selectively inhibits the production of pro-inflammatory cytokines, but enhances the expression of type I IFNs as well as IFN-stimulated genes, in response to viral infection. Our findings reveal novel dual functions of TRIM9s in antiviral immunity, which serve to balance pro-inflammatory response and production of type I IFNs.

Original languageEnglish (US)
Pages (from-to)613-628
Number of pages16
JournalCell Research
Volume26
Issue number5
DOIs
StatePublished - May 1 2016

Keywords

  • GSK3β
  • TBK1
  • TRIM9
  • ubiquitination

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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