Transposon insertional mutagenesis models of cancer

Karen M. Mann, Nancy A. Jenkins, Neal G. Copeland, Michael B. Mann

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Transposon-based insertional mutagenesis in the mouse provides a powerful approach for identifying new cancer genes. Transposon insertions in cancer genes are selected during tumor development because of their positive effect on tumor growth, and the transposon insertion sites in tumors thus serve as tags for identifying new cancer genes. Direct comparisons of transposon-mutated genes in mouse tumors with mutated genes in human tumors can lend insight into the genes and signaling pathways that drive tumorigenesis. This is critical for prioritizing genes for further study, either for their efficacy as biomarkers or drug targets. In this article, we will introduce DNA transposon-based systems used for gene discovery in mice and discuss their application to identify candidate cancer genes in light of recently published tumor studies.

Original languageEnglish (US)
Pages (from-to)235-247
Number of pages13
JournalCold Spring Harbor Protocols
Volume2014
Issue number3
DOIs
StatePublished - Mar 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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