TY - JOUR
T1 - Transplantation of clinical-grade human neural stem cells reduces neuroinflammation, prolongs survival and delays disease progression in the SOD1 rats
AU - Zalfa, Cristina
AU - Rota Nodari, Laura
AU - Vacchi, Elena
AU - Gelati, Maurizio
AU - Profico, Daniela
AU - Boido, Marina
AU - Binda, Elena
AU - De Filippis, Lidia
AU - Copetti, Massimiliano
AU - Garlatti, Valentina
AU - Daniele, Paola
AU - Rosati, Jessica
AU - De Luca, Alessandro
AU - Pinos, Francesca
AU - Cajola, Laura
AU - Visioli, Alberto
AU - Mazzini, Letizia
AU - Vercelli, Alessandro
AU - Svelto, Maria
AU - Vescovi, Angelo Luigi
AU - Ferrari, Daniela
N1 - Funding Information:
This work was supported by Italian Ministry of Health, Ricerca Corrente 2014–2017 to ALV; Association Revert Onlus, Fondazione Cellule Staminali, Fondazione Stefano Borgonovo Onlus, Assicurazioni Generali SpA, Associazione Pro Roberto, Fondazione Milan, Fondazione Cassa di RIsparmio di Terni e Narni. We would like to thank for precious technical help: Andrea Raspa (neuro surgery), Gianmarco Muzi, Claudia Ricciolini, Massimo Projetti-Pensi, Giada Sgaravizzi, (clinical-grade hNSCs production) Alessio Giavazzi, Salvatore Spano, Marta De Luca (histology).
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/5/1
Y1 - 2019/5/1
N2 - Stem cells are emerging as a therapeutic option for incurable diseases, such as Amyotrophic Lateral Sclerosis (ALS). However, critical issues are related to their origin as well as to the need to deepen our knowledge of the therapeutic actions exerted by these cells. Here, we investigate the therapeutic potential of clinical-grade human neural stem cells (hNSCs) that have been successfully used in a recently concluded phase I clinical trial for ALS patients (NCT01640067). The hNSCs were transplanted bilaterally into the anterior horns of the lumbar spinal cord (four grafts each, segments L3–L4) of superoxide dismutase 1 G93A transgenic rats (SOD1 rats) at the symptomatic stage. Controls included untreated SOD1 rats (CTRL) and those treated with HBSS (HBSS). Motor symptoms and histological hallmarks of the disease were evaluated at three progressive time points: 15 and 40 days after transplant (DAT), and end stage. Animals were treated by transient immunosuppression (for 15 days, starting at time of transplantation). Under these conditions, hNSCs integrated extensively within the cord, differentiated into neural phenotypes and migrated rostro-caudally, up to 3.77 ± 0.63 cm from the injection site. The transplanted cells delayed decreases in body weight and deterioration of motor performance in the SOD1 rats. At 40DAT, the anterior horns at L3–L4 revealed a higher density of motoneurons and fewer activated astroglial and microglial cells. Accordingly, the overall survival of transplanted rats was significantly enhanced with no rejection of hNSCs observed. We demonstrated that the beneficial effects observed after stem cell transplantation arises from multiple events that counteract several aspects of the disease, a crucial feature for multifactorial diseases, such as ALS. The combination of therapeutic approaches that target different pathogenic mechanisms of the disorder, including pharmacology, molecular therapy and cell transplantation, will increase the chances of a clinically successful therapy for ALS.
AB - Stem cells are emerging as a therapeutic option for incurable diseases, such as Amyotrophic Lateral Sclerosis (ALS). However, critical issues are related to their origin as well as to the need to deepen our knowledge of the therapeutic actions exerted by these cells. Here, we investigate the therapeutic potential of clinical-grade human neural stem cells (hNSCs) that have been successfully used in a recently concluded phase I clinical trial for ALS patients (NCT01640067). The hNSCs were transplanted bilaterally into the anterior horns of the lumbar spinal cord (four grafts each, segments L3–L4) of superoxide dismutase 1 G93A transgenic rats (SOD1 rats) at the symptomatic stage. Controls included untreated SOD1 rats (CTRL) and those treated with HBSS (HBSS). Motor symptoms and histological hallmarks of the disease were evaluated at three progressive time points: 15 and 40 days after transplant (DAT), and end stage. Animals were treated by transient immunosuppression (for 15 days, starting at time of transplantation). Under these conditions, hNSCs integrated extensively within the cord, differentiated into neural phenotypes and migrated rostro-caudally, up to 3.77 ± 0.63 cm from the injection site. The transplanted cells delayed decreases in body weight and deterioration of motor performance in the SOD1 rats. At 40DAT, the anterior horns at L3–L4 revealed a higher density of motoneurons and fewer activated astroglial and microglial cells. Accordingly, the overall survival of transplanted rats was significantly enhanced with no rejection of hNSCs observed. We demonstrated that the beneficial effects observed after stem cell transplantation arises from multiple events that counteract several aspects of the disease, a crucial feature for multifactorial diseases, such as ALS. The combination of therapeutic approaches that target different pathogenic mechanisms of the disorder, including pharmacology, molecular therapy and cell transplantation, will increase the chances of a clinically successful therapy for ALS.
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U2 - 10.1038/s41419-019-1582-5
DO - 10.1038/s41419-019-1582-5
M3 - Article
C2 - 31024007
AN - SCOPUS:85064923195
VL - 10
JO - Cell Death and Disease
JF - Cell Death and Disease
SN - 2041-4889
IS - 5
M1 - 345
ER -