Transmissive nanohole arrays for massively-parallel optical biosensing

Yanan Wang, Archana Kar, Andrew Paterson, Katerina Kourentzi, Han Le, Paul Ruchhoeft, Richard Willson, Jiming Bao

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


A high-throughput optical biosensing technique is proposed and demonstrated. This hybrid technique combines optical transmission of nanoholes with colorimetric silver staining. The size and spacing of the nanoholes are chosen so that individual nanoholes can be independently resolved in massive parallel using an ordinary transmission optical microscope, and, in place of determining a spectral shift, the brightness of each nanohole is recorded to greatly simplify the readout. Each nanohole then acts as an independent sensor, and the blocking of nanohole optical transmission by enzymatic silver staining defines the specific detection of a biological agent. Nearly 10000 nanoholes can be simultaneously monitored under the field of view of a typical microscope. As an initial proof of concept, biotinylated lysozyme (biotin-HEL) was used as a model analyte, giving a detection limit as low as 0.1 ng/mL.

Original languageEnglish (US)
Pages (from-to)241-245
Number of pages5
JournalACS Photonics
Issue number3
StatePublished - Mar 19 2014


  • biosensing
  • colorimetric detection
  • enzymatic silver staining
  • immunoassay
  • nanohole array
  • transmission optical microscope

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biotechnology
  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering


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