TY - JOUR
T1 - Translocation, biotransformation-related degradation, and toxicity assessment of polyvinylpyrrolidone-modified 2H-phase nano-MoS 2
AU - Mei, Linqiang
AU - Zhang, Xiao
AU - Yin, Wenyan
AU - Dong, Xinghua
AU - Guo, Zhao
AU - Fu, Wenhui
AU - Su, Chunjian
AU - Gu, Zhanjun
AU - Zhao, Yuliang
N1 - Funding Information:
This work was supported by the National Basic Research Programs of China (2016YFA0201600, 2015CB932104), National Natural Science Foundation of China (51772293, 51822207, 51772292, 11621505, 31571015, and 21320102003), Beijing Natural Science Foundation (2162046), and Shandong Provincial Natural Science Foundation of China (ZR2018MEE022). We gratefully acknowledge the support from Beijing Synchrotron Radiation Facility (BSRF) for SR-TXM and XANES measurements. We also thank Lirong Zheng and Wanxia Huang in BSRF during the SR-TXM and XANES measurements.
Publisher Copyright:
© 2019 The Royal Society of Chemistry.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/3/21
Y1 - 2019/3/21
N2 - Nano-MoS 2 has been extensively investigated in materials science and biomedicine. However, the effects of different methods of exposure on their translocation, biosafety, and biotransformation-related degradability remain unclear. In this study, we combined the advantages of synchrotron radiation (SR) X-ray absorption near-edge structure (XANES) and high-resolution single-cell SR transmission X-ray microscopy (SR-TXM) with traditional analytical techniques to investigate translocation, precise degraded species/ratio, and correlation between the degradation and toxicity levels of polyvinylpyrrolidone-modified 2H-phase MoS 2 nanosheets (MoS 2 -PVP NSs). These NSs demonstrated different biodegradability levels in biomicroenvironments with H 2 O 2 , catalase, and human myeloperoxidase (hMPO) (H 2 O 2 < catalase < hMPO). The effects of NSs and their biodegraded byproducts on cell viability and 3D translocation at the single-cell level were also assessed. Toxicity and translocation in mice via intravenous (i.v.), intraperitoneal (i.p.), and intragastric (i.g.) administration routes guided by fluorescence (FL) imaging were investigated within the tested dosage. After i.g. administration, NSs accumulated in the gastrointestinal organs and were excreted from feces within 48 h. After i.v. injection, NSs showed noticeable clearance due to their decreased accumulation in the liver and spleen within 30 days when compared with that in the i.p. group, which exhibited slight accumulation in the spleen. This work paves the way for understanding the biological behaviors of nano-MoS 2 using SR techniques that provide more opportunities for future applications.
AB - Nano-MoS 2 has been extensively investigated in materials science and biomedicine. However, the effects of different methods of exposure on their translocation, biosafety, and biotransformation-related degradability remain unclear. In this study, we combined the advantages of synchrotron radiation (SR) X-ray absorption near-edge structure (XANES) and high-resolution single-cell SR transmission X-ray microscopy (SR-TXM) with traditional analytical techniques to investigate translocation, precise degraded species/ratio, and correlation between the degradation and toxicity levels of polyvinylpyrrolidone-modified 2H-phase MoS 2 nanosheets (MoS 2 -PVP NSs). These NSs demonstrated different biodegradability levels in biomicroenvironments with H 2 O 2 , catalase, and human myeloperoxidase (hMPO) (H 2 O 2 < catalase < hMPO). The effects of NSs and their biodegraded byproducts on cell viability and 3D translocation at the single-cell level were also assessed. Toxicity and translocation in mice via intravenous (i.v.), intraperitoneal (i.p.), and intragastric (i.g.) administration routes guided by fluorescence (FL) imaging were investigated within the tested dosage. After i.g. administration, NSs accumulated in the gastrointestinal organs and were excreted from feces within 48 h. After i.v. injection, NSs showed noticeable clearance due to their decreased accumulation in the liver and spleen within 30 days when compared with that in the i.p. group, which exhibited slight accumulation in the spleen. This work paves the way for understanding the biological behaviors of nano-MoS 2 using SR techniques that provide more opportunities for future applications.
UR - http://www.scopus.com/inward/record.url?scp=85062872731&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85062872731&partnerID=8YFLogxK
U2 - 10.1039/c8nr10319d
DO - 10.1039/c8nr10319d
M3 - Article
C2 - 30816394
AN - SCOPUS:85062872731
SN - 2040-3364
VL - 11
SP - 4767
EP - 4780
JO - Nanoscale
JF - Nanoscale
IS - 11
ER -