Transgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons

Xin Mei Zhang; Bai Yu Chen; Alam Hoi Lam Ng; Julian A. Tanner; David Tay; Kwok Fai So; Rivka A. Rachel; Neal G. Copeland; Nancy A. Jenkins; Jian Dong Huang

doi:10.1167/iovs.04-1201

Transgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons

Xin Mei Zhang, Bai Yu Chen, Alam Hoi Lam Ng, Julian A. Tanner, David Tay, Kwok Fai So, Rivka A. Rachel, Neal G. Copeland, Nancy A. Jenkins, Jian Dong Huang

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

PURPOSE. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants. METHODS. The IRES-Cre-cDNA. fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity. RESULTS. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKCα antibody (specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina. CONCLUSIONS. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation.

Original language	English (US)
Pages (from-to)	3515-3520
Number of pages	6
Journal	Investigative Ophthalmology and Visual Science
Volume	46
Issue number	10
DOIs	https://doi.org/10.1167/iovs.04-1201
State	Published - Oct 2005

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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@article{0975d422a59d443ba2bc00ef1bdc1f32,

title = "Transgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons",

abstract = "PURPOSE. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants. METHODS. The IRES-Cre-cDNA. fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity. RESULTS. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKCα antibody (specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina. CONCLUSIONS. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation.",

author = "Zhang, {Xin Mei} and Chen, {Bai Yu} and Ng, {Alam Hoi Lam} and Tanner, {Julian A.} and David Tay and So, {Kwok Fai} and Rachel, {Rivka A.} and Copeland, {Neal G.} and Jenkins, {Nancy A.} and Huang, {Jian Dong}",

year = "2005",

month = oct,

doi = "10.1167/iovs.04-1201",

language = "English (US)",

volume = "46",

pages = "3515--3520",

journal = "Investigative Ophthalmology and Visual Science",

issn = "0146-0404",

publisher = "Association for Research in Vision and Ophthalmology Inc.",

number = "10",

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T1 - Transgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons

AU - Zhang, Xin Mei

AU - Chen, Bai Yu

AU - Ng, Alam Hoi Lam

AU - Tanner, Julian A.

AU - Tay, David

AU - So, Kwok Fai

AU - Rachel, Rivka A.

AU - Copeland, Neal G.

AU - Jenkins, Nancy A.

AU - Huang, Jian Dong

PY - 2005/10

Y1 - 2005/10

N2 - PURPOSE. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants. METHODS. The IRES-Cre-cDNA. fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity. RESULTS. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKCα antibody (specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina. CONCLUSIONS. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation.

AB - PURPOSE. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants. METHODS. The IRES-Cre-cDNA. fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity. RESULTS. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKCα antibody (specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina. CONCLUSIONS. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation.

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Transgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons

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