Transgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons

Xin Mei Zhang, Bai Yu Chen, Alam Hoi Lam Ng, Julian A. Tanner, David Tay, Kwok Fai So, Rivka A. Rachel, Neal G. Copeland, Nancy A. Jenkins, Jian Dong Huang

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

PURPOSE. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants. METHODS. The IRES-Cre-cDNA. fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity. RESULTS. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKCα antibody (specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina. CONCLUSIONS. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation.

Original languageEnglish (US)
Pages (from-to)3515-3520
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume46
Issue number10
DOIs
StatePublished - Oct 2005

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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