Transdifferentiated retinal pigment epithelial cells are immunoreactive for vascular endothelial growth factor in surgically excised age-related macular degeneration-related choroidal neovascular membranes

Pedro F. Lopez, Brian D. Sippy, H. Michael Lambert, Allen B. Thach, David R. Hinton

Research output: Contribution to journalArticle

598 Scopus citations

Abstract

Purpose. To determine the cellular origin and the vascular endothelial growth factor (VEGF) immunoreactivity of the nonvascular stromal cells in surgically excised age-related macular degeneration (ARMD)-associated choroidal neovascular membranes (CNVMs). Methods. Immunohistochemical analysis was performed on frozen sections of eight surgically excised ARMD-related CNVMs. Results. Cytokeratin-positive, smooth muscle actin-positive polygonal or fibroblastic (transdifferentiated RPE) cells were the principal nonvascular stromal cells detected. The polygonal cells were more commonly found in active (highly vascularized) regions and were strongly immunoreactive for VEGF. The fibroblastic cells were predominantly found in fibrotic (hypovascular) regions and were minimally immunoreactive for VEGF. Conclusions. Transdifferentiated RPE cells are the principal nonvascular stromal cells of both vascular and fibrotic ARMD-related CNVMs. Preferential localization of VEGF immunoreactivity within the cytoplasm of the polygonal transdifferentiated RPE cells in the highly vascularized regions of the surgically excised CNVMs suggests an important angiogenic role of these cells and this growth factor in the progression of ARMD-related choroidal neovascularization.

Original languageEnglish (US)
Pages (from-to)855-868
Number of pages14
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number5
StatePublished - 1996

Keywords

  • Age-related macular degeneration (ARMD)
  • Choroidal neovascularization (CNV)
  • Retinal pigment epithelium (RPE)
  • Transdifferentiated
  • Vascular endothelial growth factor (VEGF)

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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