Transcriptome response to nitrosative stress in Rhodobacter sphaeroides 2.4.1

Hiroyuki Arai, Jung Hyeob Roh, Jesus M. Eraso, Samuel Kaplan

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The facultative photosynthetic bacterium Rhodobacter sphaeroides 2.4.1 has a nitric oxide-response transcriptional regulator, NnrR, and nitric oxide reductase (NOR), although it is incapable of denitrification. To investigate at the genomic level the physiological response to nitrosative stress of R. sphaeroides, the transcriptome profiles of strain 2.4.1 and its NnrR mutant were analyzed before and after exposure to nitrosating agents, S-nitrosoglutathione (GSNO) and sodium nitroprusside (SNP), under microaerobic conditions. GSNO and SNP affected the expression of different but overlapping sets of genes. Only a limited number of these genes, including the genes for NOR, were under the control of NnrR, and those genes were significantly upregulated by GSNO and by SNP. The oxygen-responsive regulator FnrL and a predicted ironsensing regulator were perhaps also involved in the transcriptome response to reactive nitrogen species. Some genes, including hemN for heme biosynthesis, were subject to dual regulation by NnrR and FnrL.

Original languageEnglish (US)
Pages (from-to)111-118
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number1
DOIs
StatePublished - 2013

Keywords

  • Denitrification
  • Nitric oxide
  • NnrR
  • Rhodobacter sphaeroides
  • Transcriptome

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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