Transcription activation mediated by the bZIP factor SPA on the endosperm box is modulated by ESBF-1 in vitro

R. Steven Conlan, Michael Hammond-Kosack, Michael Bevan

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

A modified in vitro transcription system has been used to study the function of the cloned bZIP transcription factor SPA and the binding activity ESBF I in activating transcription from the bifactorial endosperm box region of the wheat prolamin LMWG-1D1 gene. Recombinant SPA expressed in Escherichia coli activated transcription from the endosperm box motif, and this was dependent upon the binding of the nuclear protein ESBF I. ESBF I did not activate transcription independently, but potentiated SPA-mediated transcriptional activation. ESBF I is likely to be the equivalent of, or contain the recently characterised DOF class of, Zn-finger protein called WPBF. These data provide new information about the interplay of members of the bZIP and DOF transcription factor families in regulating expression from bifactorial sites found in a variety of plant promoters.

Original languageEnglish (US)
Pages (from-to)173-181
Number of pages9
JournalPlant Journal
Volume19
Issue number2
DOIs
StatePublished - Jul 1999

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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