Towards the design of 3D fiber-deposited poly(ε-caprolactone)/iron-doped hydroxyapatite nanocomposite magnetic scaffolds for bone regeneration

Roberto De Santis, Alessandro Russo, Antonio Gloria, Ugo D'Amora, Teresa Russo, Silvia Panseri, Monica Sandri, Anna Tampieri, Maurilio Marcacci, Valentin A. Dediu, Colin J. Wilde, Luigi Ambrosio

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

In the past few years, researchers have focused on the design and development of three-dimensional (3D) advanced scaffolds, which offer significant advantages in terms of cell performance. The introduction of magnetic features into scaffold technology could offer innovative opportunities to control cell populations within 3D microenvironments, with the potential to enhance their use in tissue regeneration or in cell-based analysis. In the present study, 3D fully biodegradable and magnetic nanocomposite scaffolds for bone tissue engineering, consisting of a poly(ε-caprolactone) (PCL) matrix reinforced with iron-doped hydroxyapatite (FeHA) nanoparticles, were designed and manufactured using a rapid prototyping technique. The performances of these novel 3D PCL/FeHA scaffolds were assessed through a combination of theoretical evaluation, experimental in vitro analyses and in vivo testing in a rabbit animal model. The results from mechanical compression tests were consistent with FEM simulations. The in vitro results showed that the cell growth in the magnetized scaffolds was 2.2-fold greater than that in non-magnetized ones. In vivo experiments further suggested that, after only 4 weeks, the PCL/FeHA scaffolds were completely filled with newly formed bone, proving a good level of histocompatibility. All of the results suggest that the introduction of magnetic features into biocompatible materials may confer significant advantages in terms of 3D cell assembly.

Original languageEnglish (US)
Pages (from-to)1236-1246
Number of pages11
JournalJournal of Biomedical Nanotechnology
Volume11
Issue number7
DOIs
StatePublished - Jan 1 2014

Keywords

  • Bone tissue engineering
  • Experimental/theoretical analysis
  • Nanocomposite
  • Rapid prototyping
  • Scaffold

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Pharmaceutical Science

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