TY - JOUR
T1 - Toward a low-cost compact array microscopy platform for detection of tuberculosis
AU - McCall, Brian
AU - Pierce, Mark
AU - Graviss, Edward A.
AU - Richards-Kortum, Rebecca
AU - Tkaczyk, Tomasz
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011/12
Y1 - 2011/12
N2 - This paper describes the development of a microscope array capable of imaging separate fields of view without the need for opto-mechanical scanning components. This microscope array can be integrated with array illuminating optics, a full frame digital single lens reflex (DSLR) camera, and automated algorithms for the detection of Mycobacterium tuberculosis (MTB). The entire array will fit within the area of a typical sputum smear. A custom miniature objective has been designed for this microscope array that has a numerical aperture of 0.5, optical resolution of 0.63 μm, and a field of view that is 0.54 mm in diameter. A single prototype miniature objective of this design has been built, and images are presented demonstrating its imaging performance. Images are sufficiently high quality for diagnostic use. When fully integrated, this device has the potential to significantly improve performance compared to conventional microscopy systems and to enable more effective diagnosis of tuberculosis at the point of care.
AB - This paper describes the development of a microscope array capable of imaging separate fields of view without the need for opto-mechanical scanning components. This microscope array can be integrated with array illuminating optics, a full frame digital single lens reflex (DSLR) camera, and automated algorithms for the detection of Mycobacterium tuberculosis (MTB). The entire array will fit within the area of a typical sputum smear. A custom miniature objective has been designed for this microscope array that has a numerical aperture of 0.5, optical resolution of 0.63 μm, and a field of view that is 0.54 mm in diameter. A single prototype miniature objective of this design has been built, and images are presented demonstrating its imaging performance. Images are sufficiently high quality for diagnostic use. When fully integrated, this device has the potential to significantly improve performance compared to conventional microscopy systems and to enable more effective diagnosis of tuberculosis at the point of care.
KW - Array microscopy
KW - Fluorescence microscopy
KW - Microscopic observation drug susceptibility
KW - Miniature objective
KW - Sputum smear microscopy
UR - http://www.scopus.com/inward/record.url?scp=84655162151&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84655162151&partnerID=8YFLogxK
U2 - 10.1016/j.tube.2011.10.011
DO - 10.1016/j.tube.2011.10.011
M3 - Article
C2 - 22079590
AN - SCOPUS:84655162151
VL - 91
SP - S54-S60
JO - Tuberculosis
JF - Tuberculosis
SN - 1472-9792
IS - SUPPL. 1
ER -