@inbook{05f697d12d8d4418a2913139795b69d8,
title = "Total internal reflection fluorescence (TIRF) microscopy for real-time imaging of nanoparticle-cell plasma membrane interaction",
abstract = "Nanoparticulate systems are widely used for site-specific drug and gene delivery as well as for medical imaging. The mode of nanoparticle-cell interaction may have a significant effect on the pathway of nanoparticle internalization and subsequent intracellular trafficking. Total internal reflection fluorescence (TIRF) microscopy allows for real-time monitoring of nanoparticle-membrane interaction events, which can provide vital information in relation to design and surface engineering of therapeutic nanoparticles for cell-specific targeting. In contrast to other microscopy techniques, the bleaching effect by lasers in TIRF microscopy is considerably less when using fluorescent nanoparticles and it reduces photo-induced cytotoxicity during visualization of live-cell events since it only illuminates the specific area near or at the plasma membrane.",
keywords = "Cell surface, Live-cell imaging, Nano-particles, TIRFM, Trafficking, Widefield microscopy",
author = "Ladan Parhamifar and Seyed Moghimi",
year = "2012",
month = aug,
day = "15",
doi = "10.1007/978-1-61779-953-2_38",
language = "English (US)",
isbn = "9781617799525",
series = "Methods in Molecular Biology",
pages = "473--482",
editor = "Mikhail Soloviev",
booktitle = "Nanoparticles in Biology and Medicine",
}