Toki-shakuyaku-san, a Japanese kampo medicine, reduces colon inflammation in a mouse model of acute colitis

Remya Sreedhar, Somasundaram Arumugam, Rajarajan A. Thandavarayan, Vijayasree V. Giridharan, Vengadeshprabhu Karuppagounder, Vigneshwaran Pitchaimani, Rejina Afrin, Meilei Harima, Takashi Nakamura, Kazuyuki Ueno, Masahiko Nakamura, Kenji Suzuki, Kenichi Watanabe

    Research output: Contribution to journalArticlepeer-review

    15 Scopus citations


    Toki-shakuyaku-san (TOKI) is a Japanese kampo medicine, which consists of a mixture of herbal medicines and considered to be a promising remedial agent due to its immunomodulatory and anti-inflammatory effects. We examined the beneficial effects of TOKI in inflammatory bowel disease associated with the inflammation of the intestinal barrier. A study was designed, using C57BL/6 female mice and were administered with 3% DSS in drinking water for 8 days with or without 1 g/kg/day TOKI orally for the last 3 days and a normal group supplied with plain drinking water for 8 days. TOKI treatment attenuated the clinical symptoms of acute murine colitis and also alleviated the inflammatory mechanism by reducing the inflammatory mediators, such as IL-1β, IL-2, TGF-β, RAGE and TLR2. It has also decreased the levels of CHOP, caspase12, cleaved caspase3 and cleaved caspase7 and thereby down-regulated the endoplasmic reticulum stress and apoptotic signaling induced by DSS. Moreover, the expression levels of cyclin D1 and c-kit have also confirmed the beneficial role of TOKI in colitis. All these data suggested that TOKI can be a promising agent for the treatment of colitis since it alleviates the disease progression and severity.

    Original languageEnglish (US)
    Pages (from-to)869-875
    Number of pages7
    JournalInternational Immunopharmacology
    Issue number2
    StatePublished - Dec 1 2015


    • Apoptosis
    • Endoplasmic reticulum stress
    • Inflammation
    • Inflammatory bowel diseases
    • Kampo medicine
    • Toki-shakuyaku-san

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology
    • Pharmacology


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