TY - JOUR
T1 - TLR6
T2 - A novel member of an expanding Toll-like receptor family
AU - Takeuchi, O.
AU - Kawai, T.
AU - Sanjo, H.
AU - Copeland, N. G.
AU - Gilbert, D. J.
AU - Jenkins, N. A.
AU - Takeda, K.
AU - Akira, S.
N1 - Funding Information:
We thank T. Aoki and M. Hyuga for excellent secretarial assistance, Andreé Reuss for excellent technical assistance. This work was supported by grants from the Ministry of Education of Japan and in part by the National Cancer Institute, DHHS, under contract with ABL. The nucleotide sequence data reported in this paper will appear in the DDBJ, EMBL and GenBank nucleotide sequence databases with Accession Nos AB020807 (human TLR6) and AB020808 (murine TLR6).
PY - 1999/4/29
Y1 - 1999/4/29
N2 - Drosophila Toll protein is shown to activate the innate immune system in adult and regulate the dorsoventral patterning in the developing embryo. Recently, five human homologs of Drosophila Toll, designated as Toll-like receptors (TLRs), have been identified and shown to play a role in the innate immune response. We report here the molecular cloning and characterization of a new member of Toll-like receptor family, Toll-like receptor 6 (TLR6). Human and murine TLR6 are type-I transmembrane receptors that contain both an extracellular leucine-rich repeat (LRR) domain and a cytoplasmic Toll/IL-1 receptor (IL-1R)-like region. The amino acid sequence of human TLR6 (hTLR6) is most similar to that of hTLR1 with 69% identity. RT-PCR analysis revealed that murine TLR6 is expressed predominantly in spleen, thymus, ovary and lung. Like other TLR family members, constitutively active TLR6 activates both NF-κB and c-Jun N-terminal kinase (JNK). The TLR6 gene, as well as the TLR1 gene, mapped to the proximal region of murine chromosome 5 within 1.7 cM of each other. These results suggest that TLR6 is a novel member of an expanding TLR family.
AB - Drosophila Toll protein is shown to activate the innate immune system in adult and regulate the dorsoventral patterning in the developing embryo. Recently, five human homologs of Drosophila Toll, designated as Toll-like receptors (TLRs), have been identified and shown to play a role in the innate immune response. We report here the molecular cloning and characterization of a new member of Toll-like receptor family, Toll-like receptor 6 (TLR6). Human and murine TLR6 are type-I transmembrane receptors that contain both an extracellular leucine-rich repeat (LRR) domain and a cytoplasmic Toll/IL-1 receptor (IL-1R)-like region. The amino acid sequence of human TLR6 (hTLR6) is most similar to that of hTLR1 with 69% identity. RT-PCR analysis revealed that murine TLR6 is expressed predominantly in spleen, thymus, ovary and lung. Like other TLR family members, constitutively active TLR6 activates both NF-κB and c-Jun N-terminal kinase (JNK). The TLR6 gene, as well as the TLR1 gene, mapped to the proximal region of murine chromosome 5 within 1.7 cM of each other. These results suggest that TLR6 is a novel member of an expanding TLR family.
KW - JNK
KW - NF-κB
KW - Toll-like receptor family
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U2 - 10.1016/S0378-1119(99)00098-0
DO - 10.1016/S0378-1119(99)00098-0
M3 - Article
C2 - 10231569
AN - SCOPUS:0033614428
VL - 231
SP - 59
EP - 65
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1-2
ER -