Time-resolved fluorescence measurements using stroboscopic excitation

Daniel R. Matthews; Huw D. Summers; Kerenza Njoh; Rachel J. Errington; Paul J. Smith; Simon Ameer-Beg; Boris Vojnovic

Time-resolved fluorescence measurements using stroboscopic excitation

Daniel R. Matthews, Huw D. Summers, Kerenza Njoh, Rachel J. Errington, Paul J. Smith, Simon Ameer-Beg, Boris Vojnovic

Houston Methodist

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Scopus citations

Abstract

We report on the development of a simple technique for obtaining time-domain information using dc detection of fluorescence. We show that this is feasible for assays where a change in lifetime of an indicator occurs in reaction to an analyte, in fluorescence resonance energy transfer for example, and could be particularly useful for assays performed in the scaled-down environment of a "lab-on-a-chip", A rate equation model is presented which allows an objective analysis of the relative importance of the key measurement parameters: optical saturation of the fluorophore and excitation pulse characteristics. We present a comparison of the model with a cuvette based analysis of a carbocyanine dye where the excitation source is a 650 nm wavelength, self-pulsing AlGaInP laser diode.

Original language	English (US)
Title of host publication	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Editors	C.D. Depeursinge
Pages	1-7
Number of pages	7
Volume	5864
State	Published - 2005
Event	Novel Optical Instrumentation for Biomedical Applications II - Munich, Germany Duration: Jun 12 2005 → Jun 16 2005

Other

Other	Novel Optical Instrumentation for Biomedical Applications II
Country/Territory	Germany
City	Munich
Period	6/12/05 → 6/16/05

ASJC Scopus subject areas

Engineering(all)

Cite this

Matthews, DR, Summers, HD, Njoh, K, Errington, RJ, Smith, PJ, Ameer-Beg, S & Vojnovic, B 2005, Time-resolved fluorescence measurements using stroboscopic excitation. in CD Depeursinge (ed.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 5864, 586409, pp. 1-7, Novel Optical Instrumentation for Biomedical Applications II, Munich, Germany, 6/12/05.

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title = "Time-resolved fluorescence measurements using stroboscopic excitation",

abstract = "We report on the development of a simple technique for obtaining time-domain information using dc detection of fluorescence. We show that this is feasible for assays where a change in lifetime of an indicator occurs in reaction to an analyte, in fluorescence resonance energy transfer for example, and could be particularly useful for assays performed in the scaled-down environment of a {"}lab-on-a-chip{"}, A rate equation model is presented which allows an objective analysis of the relative importance of the key measurement parameters: optical saturation of the fluorophore and excitation pulse characteristics. We present a comparison of the model with a cuvette based analysis of a carbocyanine dye where the excitation source is a 650 nm wavelength, self-pulsing AlGaInP laser diode.",

author = "Matthews, {Daniel R.} and Summers, {Huw D.} and Kerenza Njoh and Errington, {Rachel J.} and Smith, {Paul J.} and Simon Ameer-Beg and Boris Vojnovic",

year = "2005",

language = "English (US)",

volume = "5864",

pages = "1--7",

editor = "C.D. Depeursinge",

booktitle = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

note = "Novel Optical Instrumentation for Biomedical Applications II ; Conference date: 12-06-2005 Through 16-06-2005",

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TY - GEN

T1 - Time-resolved fluorescence measurements using stroboscopic excitation

AU - Matthews, Daniel R.

AU - Summers, Huw D.

AU - Njoh, Kerenza

AU - Errington, Rachel J.

AU - Smith, Paul J.

AU - Ameer-Beg, Simon

AU - Vojnovic, Boris

PY - 2005

Y1 - 2005

N2 - We report on the development of a simple technique for obtaining time-domain information using dc detection of fluorescence. We show that this is feasible for assays where a change in lifetime of an indicator occurs in reaction to an analyte, in fluorescence resonance energy transfer for example, and could be particularly useful for assays performed in the scaled-down environment of a "lab-on-a-chip", A rate equation model is presented which allows an objective analysis of the relative importance of the key measurement parameters: optical saturation of the fluorophore and excitation pulse characteristics. We present a comparison of the model with a cuvette based analysis of a carbocyanine dye where the excitation source is a 650 nm wavelength, self-pulsing AlGaInP laser diode.

AB - We report on the development of a simple technique for obtaining time-domain information using dc detection of fluorescence. We show that this is feasible for assays where a change in lifetime of an indicator occurs in reaction to an analyte, in fluorescence resonance energy transfer for example, and could be particularly useful for assays performed in the scaled-down environment of a "lab-on-a-chip", A rate equation model is presented which allows an objective analysis of the relative importance of the key measurement parameters: optical saturation of the fluorophore and excitation pulse characteristics. We present a comparison of the model with a cuvette based analysis of a carbocyanine dye where the excitation source is a 650 nm wavelength, self-pulsing AlGaInP laser diode.

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UR - http://www.scopus.com/inward/citedby.url?scp=28844478457&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:28844478457

VL - 5864

SP - 1

EP - 7

BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

A2 - Depeursinge, C.D.

T2 - Novel Optical Instrumentation for Biomedical Applications II

Y2 - 12 June 2005 through 16 June 2005

ER -

Time-resolved fluorescence measurements using stroboscopic excitation

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