Time-resolved fluorescence measurements using stroboscopic excitation

Daniel R. Matthews, Huw D. Summers, Kerenza Njoh, Rachel J. Errington, Paul J. Smith, Simon Ameer-Beg, Boris Vojnovic

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

We report on the development of a simple technique for obtaining time-domain information using dc detection of fluorescence. We show that this is feasible for assays where a change in lifetime of an indicator occurs in reaction to an analyte, in fluorescence resonance energy transfer for example, and could be particularly useful for assays performed in the scaled-down environment of a "lab-on-a-chip", A rate equation model is presented which allows an objective analysis of the relative importance of the key measurement parameters: optical saturation of the fluorophore and excitation pulse characteristics. We present a comparison of the model with a cuvette based analysis of a carbocyanine dye where the excitation source is a 650 nm wavelength, self-pulsing AlGaInP laser diode.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsC.D. Depeursinge
Pages1-7
Number of pages7
Volume5864
StatePublished - 2005
EventNovel Optical Instrumentation for Biomedical Applications II - Munich, Germany
Duration: Jun 12 2005Jun 16 2005

Other

OtherNovel Optical Instrumentation for Biomedical Applications II
CountryGermany
CityMunich
Period6/12/056/16/05

ASJC Scopus subject areas

  • Engineering(all)

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